Octanol/water partitioning simulation by reversed-phase high performance liquid chromatography for structurally diverse acidic drugs: Effect of n-octanol as mobile phase additive

The role of n-octanol as mobile phase additive for the lipophilicity assessment of 45 structurally diverse acidic drugs both at neutral (pH 2.5) and ionized form (pH 7.4) was investigated. Extrapolated retention factors log kw were determined on a BDS C18 column using methanol as organic modifier and different amounts of n-octanol as mobile phase additive. For more polar compounds, the effect of n-octanol in retention was found to decrease as...