Purification and Characterization of Human Laminin-8

Recently identified laminin isoforms containing the α4 chain have been shown to be expressed in the basement membrane of restricted organs such as heart, skeletal muscle, and blood vessels, especially those in embryos. We screened 38 human cell lines for the expression of the laminin α4 chain by reverse transcriptase-polymerase chain reaction and found that T98G glioblastoma cells express only α4, but not other α chains. Laminin-8, an isoform...