Journal of Biological Chemistry
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#1Valentina A. Spiteri (UCL: University College London)
#2Margaret Goodall (University of Birmingham)H-Index: 31
Last. Stephen J. Perkins (UCL: University College London)H-Index: 63
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Human immunoglobulin G subclass 3 (IgG3) possesses a uniquely long hinge region that separates its Fab antigen-binding and Fc receptor-binding regions. Owing to this hinge length, the molecular structure of full-length IgG3 remains elusive, and the role of the two conserved Fc glycosylation sites are unknown. To address these issues, we subjected glycosylated and deglycosylated human myeloma IgG3 to multidisciplinary solution structure studies. Using analytical ultracentrifugation, the elongated...
#1Sai Shi (HEBUT: Hebei University of Technology)H-Index: 5
#2Biao Ma (HEBUT: Hebei University of Technology)H-Index: 3
Last. Hailong An (HEBUT: Hebei University of Technology)H-Index: 11
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As a calcium-activated chloride channel regulated by the intracellular Ca2+ concentration and membrane potential, TMEM16A has attracted considerable attention and has been proposed as a novel anticancer drug target. We have previously reported that the pocket above the ion conductance pore could be a nonselective inhibitor-binding pocket. However, whether this pocket is druggable remains unexplored. In this study, we performed virtual screening to target the presumed inhibitor-binding pocket and...
#1Tatsuya Kondo (OPU: Osaka Prefecture University)
#2Miyu Kichijo (OPU: Osaka Prefecture University)
Last. Tatsuji Sakamoto (OPU: Osaka Prefecture University)H-Index: 22
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Gum arabic (GA) is widely used as an emulsion stabilizer and coating in several industrial applications, such as foods and pharmaceuticals. GA contains a complex carbohydrate moiety, and the nonreducing ends of the side chains are often capped with l-rhamnose; thus, enzymes that can remove these caps are promising tools for the structural analysis of the carbohydrates comprising GA. In this study, GA-specific l-rhamnose-α-1,4-d-glucuronate lyase from the fungus Fusarium oxysporum 12S (FoRham1) w...
#1Xundong Wei (CAS: Chinese Academy of Sciences)H-Index: 3
#2Jianhua Zhang (CAS: Chinese Academy of Sciences)H-Index: 101
Last. Jie Ma (CAS: Chinese Academy of Sciences)
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Abstract null null The Split-Cre system is a powerful tool for genetic manipulation and can be used to spatiotemporally control gene expression in vivo. However, the low activity of the reconstituted NCre/CCre recombinase in the Split-Cre system limits its application as an indicator of the simultaneous expression of a pair of genes of interest. Here, we describe two approaches for improving the activity of the Split-Cre system after Cre reconstitution based on self-associating split green fluor...
#1Kohji Mori (Osaka University)H-Index: 17
#2Shiho Gotoh (Osaka University)H-Index: 1
Last. Christian Haass (German Center for Neurodegenerative Diseases)H-Index: 136
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Abstract null GGGGCC (G4C2) repeat expansion in the C9orf72 gene has been shown to cause frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS). Dipeptide repeat proteins (DPR) produced through repeat-associated non-AUG (RAN) translation are recognized as potential drivers for neurodegeneration. Therefore, selective inhibition of RAN translation could be a therapeutic avenue to treat these neurodegenerative diseases. It was previously known that the porphyrin TMPyP4 bin...
#1Lishan Zhang (SYSU: Sun Yat-sen University)H-Index: 1
#2Shanhui Ge (SYSU: Sun Yat-sen University)H-Index: 2
Last. Mian Zeng (SYSU: Sun Yat-sen University)H-Index: 5
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Abstract null null Pulmonary endothelial barrier dysfunction is a major pathophysiology observed in acute respiratory distress syndrome (ARDS). Ghrelin, a key regulator of metabolism, has been shown to play protective roles in the respiratory system. However, its effects on lipopolysaccharide (LPS)-induced pulmonary endothelial barrier injury are unknown. In this study, the effects of ghrelin on LPS-induced ARDS and endothelial cell injury were evaluated in vivo and in vitro. In vivo, mice treat...
#1Jean-Pierre Vilardaga (University of Pittsburgh)H-Index: 44
#2Ieva Sutkeviciute (University of Pittsburgh)H-Index: 13
Last. Karina Peña (University of Pittsburgh)H-Index: 6
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Abstract null null Cyclic AMP (cAMP) is the indispensable second messenger regulating cell metabolism and function in response to extracellular hormones and neurotransmitters. cAMP is produced via the activation of G protein–coupled receptors (GPCRs) located at both the cell surface and inside the cell. Recently, Tsvetanova et al. explored cAMP generation in distinct locations and the impact on respective cell functions. Using a phospho-proteomic analysis, they provide insight into the unique ro...
#1Michael J. Currie (Cant.: University of Canterbury)H-Index: 1
#2Lavanyaa ManjunathH-Index: 2
Last. Renwick C. J. Dobson (Cant.: University of Canterbury)H-Index: 32
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There are five known general catalytic mechanisms used by enzymes to catalyze carbohydrate epimerization. The amino sugar epimerase N-acetylmannosamine-6-phosphate 2-epimerase (NanE) has been proposed to use a deprotonation-reprotonation mechanism, with an essential catalytic lysine required for both steps. However, the structural determinants of this mechanism are not clearly established. We characterized NanE from Staphylococcus aureus (SaNanE) using a new coupled assay to monitor NanE catalys...
#1Robbins Puthenveetil (NIH: National Institutes of Health)
#2Cheng man Lun (NIH: National Institutes of Health)
Last. Anirban Banerjee (NIH: National Institutes of Health)H-Index: 31
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Abstract null null S-acylation, also known as palmitoylation, is the most widely prevalent form of protein lipidation, whereby long chain fatty acids get attached to cytosol-facing cysteines. In humans, 23 members of the zDHHC family of integral membrane enzymes catalyze this modification. S-acylation is critical for the life cycle of many enveloped viruses. The Spike protein of SARS-CoV-2, the causative agent of COVID-19, has the most cysteine-rich cytoplasmic tail among known human pathogens i...
#1Michael Salsaa (WSU: Wayne State University)H-Index: 3
#2Kerestin Aziz (WSU: Wayne State University)
Last. Miriam L. Greenberg (WSU: Wayne State University)H-Index: 49
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Valproate (VPA) is a widely used mood stabilizer, but its therapeutic mechanism of action is not understood. This knowledge gap hinders the development of more effective drugs with fewer side effects. Using the yeast model to elucidate the effects of VPA on cellular metabolism, we determined that the drug upregulated expression of genes normally repressed during logarithmic growth on glucose medium and increased levels of activated (phosphorylated) Snf1 kinase, the major metabolic regulator of t...
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