Application and detection of (14)c-hd in two mouse models.

Published on Jan 1, 2002in Toxicology Mechanisms and Methods2.295
· DOI :10.1080/15376520208951165
Thomas P. Logan2
Estimated H-index: 2
(United States Army Medical Research Institute of Chemical Defense),
Thomas P. Logan6
Estimated H-index: 6
(United States Army Medical Research Institute of Chemical Defense)
+ 3 AuthorsRobert P. Casillas20
Estimated H-index: 20
(United States Army Medical Research Institute of Chemical Defense)
Sources
Abstract
The CD1-haired mouse and the SKH-hairless mouse are two animal models that have been used to evaluate sulfur mustard (HD) exposure and protection in our laboratory. In a recent study we observed that a substance P inhibitor protected the haired mouse ear against an HD solution, but the same drug was not successful in protecting the hairless mouse against HD vapor. This experiment prompted us to compare HD exposures between these models. We determined the 14C content in the skin after exposures to HD containing 14C-HD. Rate curves were generated for applications of (1) HD in methylene chloride to the haired mouse ear; (2) HD in methylene chloride to the hairless mouse dorsal skin; and (3) saturated HD vapor to the hairless mouse dorsal skin for 6 min. The curves showed a reduction in 14C disintegrations per min in animals euthanized 0 to 2 h postexposure. The largest percentage of decrease of 14C content in skin occurred within 30 min of HD challenge for all exposures. An 8-mm skin-punch biopsy and a 14-mm...
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ABSTRACTThe hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD), bis(2-chloroethylsulfide), exposure. We determined the HD content in the skin of HGP after a 7-min exposure to vapors saturated with a mixture of HD and l4C-HD. Concentration/time (CT) values in the range of 2 μg/cm2/min were determined by counting skin 14C disintegrations per min (dpm) in animals euthanized immediately after exposure. These values are similar to human pe...
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