Original paper
Improved vectors and genome-wide libraries for CRISPR screening
Abstract
Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and have been used to reveal new mechanisms in diverse biological models1-4. Previously, we used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations conferring...
Paper Details
Title
Improved vectors and genome-wide libraries for CRISPR screening
Published Date
Jul 30, 2014
Journal
Volume
11
Issue
8
Pages
783 - 784
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Notes
History