Sensitive ELISA Method for the Measurement of Catabolites of Antibody–Drug Conjugates (ADCs) in Target Cancer Cells

A new, sensitive ELISA method has been developed which measures catabolites in cells and media upon processing of antibody–drug conjugates (ADCs) by target cancer cells. This ELISA method, exemplified for maytansinoid ADCs, uses competitive inhibition by a maytansinoid analyte of the binding of biotinylated antimaytansine antibody to an immobilized BSA–maytansinoid conjugate. Synthetic standards of several maytansinoid catabolites derived from...