: The present experiments were undertaken to clarify the differences in humoral and cellular immune responses to a low-molecular compound, sodium 2,4,6-trinitrobenznnesul fonate dihydrate (TNBS) in guinea-pig strains. Guinea pigs of three different strains, Hartley, Strain 2 and Strain 13, were immunized subcutaneously with TNBS (3 mg/body) 2 or 3 times a week, 9 times in total. Humoral immune responses to TNBS were assessed by passive cutaneous anaphylaxis (PCA) and Arthus reaction, and cellular immune response was assessed by delayed-type hypersensitivity (DTH). Hartley guinea pigs showed high humoral immune responses to TNBS, whereas Strain 2 and 13 guinea pigs showed low responses. Strain 2 guinea pigs displayed high cellular immune response to TNBS, and Strain 13 displayed low cellular immune responses. These results suggest that the pattern of humoral immune response to TNBS does not correlate with that of the cellular immune responses to TNBS for Strain 2 and Hartley guinea pigs.
Optimal experimental methods for antigenicity studies in guinea pigs were investigated on : (1) the effects of different immunizing methods using complete or incomplete Freund's adjuvants (CFA or IFA), and various injection sites, the number of immunizations, the immunizing doses, and the immunizing periods, (2) the relationship between the severity of active systemic anaphylaxis (ASA) reactions and passive cutaneous anaphylaxis (PCA) titers, (3) positive control for oral administration, and (4)...
#1François Verdier(French Institute of Health and Medical Research)H-Index: 13
#2Isabelle Chazal(French Institute of Health and Medical Research)H-Index: 1
Last. Jacques Descotes(French Institute of Health and Medical Research)H-Index: 30
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Abstract Immediate hypersensitivity (or anaphylactic) reactions can be caused by large molecules which are directly immunogenic or by smaller molecules which bind to macromolecular carriers and act as haptens. To reproduce these reactions in animals, either systemic or local models are used in actively or passively sensitized animals, respectively. Several attempts have been made to detect the potential of new drugs and chemicals to induce anaphylactic reactions. Protocols using the inhalation o...
: The present studies were undertaken to clarify the difference of the immunogenicity evoked in rabbits immunized with a low-molecular chemical antigen (TNBS) alone, by four administration routes. Rabbits were divided into four experimental groups: TNBS sc group, i.v. group, im group and ip group (3 times/week, 10 times in total), and immune responses to TNBS were estimated by the assay systems of humoral immunoreactions (HA.PCA.Arthus) and cellular immunoreaction (DTH). The highest evoking of t...
In order to develop a system for evaluating the allergenicity of drugs in clinical use, we tested drugs for the ability to induce drug-specific immune responses in guinea pigs and mice. Test drugs were benzylpenicillin, procainamide, hydralazine, isoniazid, α-methyldopa, D-penicillamine, captopril, sulfamethoxazole and 2, 4-dinitrochlorobenzene (DNCB), which are known to induce allergic responses in man including hypersensitivity reactions and drug-induced auto-immune responses. Guinea pigs were...
The present studies were undertaken to clarify the immunogenicity evoked in four species, the guinea pig, rabbit, F344 rat and A/J mouse, immunized with a low-molecular antigen (TNBS) alone, TNBS plus Alum and TNBS plus FCA. Each species was divided into three groups: TNBS alone sc group, TNBS plus Alum ip groups (one and 3 times sensitization) and TNBS plus FCA sc groups (one and 3 times sensitization), and immune responses to TNBS were estimated by the assay systems of humoral immunoreactions ...
: It appears to be significant for the analytical studies of drug allergy that a comparison is made among inbred strains of mice, with different H-2 haplotypes with respect to the antibody response to low-molecular compounds. Such studies have been demonstrated by several investigators using high-molecular antigens, such as the synthetic polypeptides and natural proteins. The present experiments were undertaken, therefore, to clarify the differences in antibody responses to a low-molecular compo...
We have examined the immunological activity of five affinity-purified protein antigens from Mycobacterium tuberculosis in seven inbred and one outbred guinea pig strains. The test systems were measurements of delayed-type hypersensitivity (Dth) responses, lymphocyte stimulation assays (LS), and antibody response measurements. The results showed significant differences in the immunogenicity of the single-protein antigens and, when the antigens were considered separately, highly significant guinea...
: Antigenicity of penicillin G (PCG) was studied in guinea pigs. PCG 5 mg, 10 mg or 25 mg with Freund's complete adjuvant each on days 0, 7 and 21 was injected to a guinea pig: intramuscularly into both thighs and intracutaneously into four locations on the back. A remarkable antigenicity was induced in animals immunized with 25 mg although only low antigenicity in 5 mg and 10 mg. A maximum serum level of the antibody was observed about 2 weeks after last immunization and all of animals immunize...
: Experiments were carried out to determine the cause of individual differences in the passive cutaneous anaphylaxis (PCA) response in guinea pigs. The intensity of 4-h homologous PCA produced by anti-penicillin G serum was not markedly different among eight reactive sites on the back of a specified animal, whereas considerable individual differences were observed in the PCA response, even at a specified reactive site. PCA was significantly inhibited by an antihistaminic agent, promethazine, and...
#1Troy C. Sutton(UBC: University of British Columbia)H-Index: 18
#2Farnoosh Tayyari(UBC: University of British Columbia)H-Index: 6
Last. Richard G. Hegele(UBC: University of British Columbia)H-Index: 13
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T Helper 1 Background Protects Against Airway Hyperresponsiveness and Inflammation in Guinea Pigs With Persistent Respiratory Syncytial Virus Infection
In the present study, the correlation between mouse PLNA and those obtained from GP-PCA and from GP-ASA reactions fro sodium 2, 4, 6-trinitrobenzenesulfonate dihydrate (TNBS), penicillin G and cephalothin was investigated. Next, variou drugs were tested using the mouse PLNA to study whether PLN reactivity could be related to the potential of the compounds to induce allergic and autoimmune disorders in humans. The parameter of the PLNA was determined by the PLN cellularity index in BALB/c and A/J...
Guinea pigs of two strains, outbred Hartley and inbred Strain 2, were immunized sub-cutaneously with cephalothin (CET, 20 mg/body) alone, without adjuvant. Immune responses to the antibiotic were assessed by guinea-pig passive cutaneous anaphylaxis (GP-PCA) and active systemic anaphylaxis (GP-ASA) reactions. The immune response to CET in the female Hartley guinea pigs was higher than that in the males. In contrast, no difference in response to CET in Strain 2 guinea pigs was observed between mal...
The present experiments were undertaken to clarify the differences in humoral immune response to two β-lactam antibiotics, benzylpenicillin potassium (PcG) and cephalothin sodium (CET), in guinea-pig strains. Guinea pigs of three different strains, Hartley, Strain 2 and Strain 13, were immunized subcutaneously with PcG (10mg/body) 1 or 3 times a week, 10 times in total, or with CET (20 mg/body) 3 times a week, 12 times in total. Humoral immune responses to the two antibiotics were assessed by pa...