Preparation of a novel porous scaffold from poly(lacticco-glycolic acid)/hydroxyapatite

Published on Jul 21, 2011in Asian Biomedicine0.324
· DOI :10.5372/ABM.V5I4.734
Masoume Haghbin Nazarpak3
Estimated H-index: 3
Background: Scaffolds for bone tissue engineering must meet functional requirements, porosity, biocompatibility, and biodegradability. Different polymeric scaffolds have been designed to satisfy these properties. Composite materials could improve mechanical properties compared with polymers, and structural integrity and flexibility compared with brittle ceramics. Objective: Fabricate poly (lactic-co-glycolic acid) (PLGA) /hydroxyapatite (HA) porous scaffolds by freezeextraction method, and evaluate the possibility for optimizing their biocompatibility by changing their HA content. Methods: Porous PLGA/HA composites structure were prepared by freezing a polymer solution, and then the solvent was extracted by a non-solvent and subsequently air-dried. The scaffolds were coated with triblock copolymer and sterilized by ultraviolet light. Human mesenchymal stem cells were cultured on the prepared scaffolds and were studied after three days by 4, 6-diamidino-2-phenylindole (DAPI) fluorescence microscopy. Results: Microstructural studies with SEM showed the formation of about 50 micrometer size porous structure and interconnected porosity so that cells were adhered well into the structure of the coated samples. DAPI fluorescence microscopy showed more cell adhesion to the coated scaffolds and cell diffusion into the pores are visible. Direct assay of cell proliferation performed with MTT test showed cell growing on the scaffold similar to or more than on control samples. Conclusion: The triblock-coated PLGA/HA porous scaffolds may provide cell adhesion and proliferation, demonstrating their potential application in bone engineering. Keywords: Cell adhesion, composite, freeze-extraction, human mesenchymal stem cells (hMSC), porous scaffold, tissue engineering
📖 Papers frequently viewed together
3 Citations
3 Citations
10 Citations
#1Therese Tyson (KTH: Royal Institute of Technology)H-Index: 3
#2Anna Finne-WistrandH-Index: 31
Last. Ann-Christine AlbertssonH-Index: 81
view all 3 authors...
A simple and effective method of fabricating scaffolds with open pore structures was successfully used on several copolymers. The method, which is straightforward and fast, was developed to overcome problems such as low pore interconnectivity and to achieve thick three-dimensional scaffolds. Copolymers are of particular interest because it is possible to tune their mechanical and degradable properties, and in this work, copolymers of l-lactide (LLA) and trimethylene carbonate (TMC) were synthesi...
61 CitationsSource
#1Frank Lyons (RCSI: Royal College of Surgeons in Ireland)H-Index: 7
#2Sonia Partap (RCSI: Royal College of Surgeons in Ireland)H-Index: 8
Last. Fergal J. O'Brien (RCSI: Royal College of Surgeons in Ireland)H-Index: 80
view all 3 authors...
25 CitationsSource
#1Xinli Zhu (Southwest Jiaotong University)H-Index: 4
#2Wenguo CuiH-Index: 51
Last. Yan JinH-Index: 3
view all 4 authors...
Diffusional limitations of mass transport have adverse effects on engineering tissues that normally have high vascularity and cellularity. The current electrospinning method is not always successful to create micropores to encourage cell infiltration within the scaffold, especially when relatively large-sized pores are required. In this study, a slow rotating frame cylinder was developed as the collector to extend the pore size and increase the porosity of electrospun fibrous scaffolds. Fibrous ...
325 CitationsSource
#1Yan Qi Goh (NTU: Nanyang Technological University)H-Index: 1
#2Chui Ping Ooi (NTU: Nanyang Technological University)H-Index: 1
Freeze-extraction, which involves phase separation principle, gave highly porous scaffolds without the time and energy consuming freeze-drying process. The presented method eliminates the problem of formation of surface skin observed in freeze-drying methods. The effects of different freezing temperature (−80 and −24°C), medium (dry ice/ethanol bath and freezer) and polymer concentrations (1, 3, and 5 wt.%) on the scaffold properties were investigated in connection with the porous morphology and...
59 CitationsSource
#1Quynh Pham (Rice University)H-Index: 15
#2Upma SharmaH-Index: 13
Last. Antonios G. MikosH-Index: 153
view all 3 authors...
The physical and spatial architectural geometries of electrospun scaffolds are important to their application in tissue engineering strategies. In this work, poly(e-caprolactone) microfiber scaffolds with average fiber diameters ranging from 2 to 10 μm were individually electrospun to determine the parameters required for reproducibly fabricating scaffolds. As fiber diameter increased, the average pore size of the scaffolds, as measured by mercury porosimetry, increased (values ranging from 20 t...
905 CitationsSource
#1L. Mathieu (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 8
#2Pierre-Etienne Bourban (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 31
Last. Jan-Anders E. Månson (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 68
view all 3 authors...
Three methods to mix ceramic fillers, hydroxyapatite or β-tricalcium phosphate, with a polymer matrix, a poly l-lactic acid, are investigated as a first step prior to supercritical foaming to prepare porous composite structures for biomedical applications. First the dry process consists in mixing ceramic powder and polymer pellets before a compression molding step. The second technique is based on the dispersion of ceramic fillers into a polymer–solvent solution. The third method is a melt extru...
105 CitationsSource
The state of the art of calcium phosphate/polymer composites for bone substitution is reviewed. Many combinations are possible which were proposed to improve the mechanical properties and the biocompatibility. However, the way from the laboratory to the clinical application is long, and potential candidates for new bone substitution materials have to meet many different requirements.
84 CitationsSource
#1Dietmar W. Hutmacher (NUS: National University of Singapore)H-Index: 114
#2Andrés J. García (Georgia Institute of Technology)H-Index: 91
The first generation of clinically applied tissue engineering concepts in the area of skin, cartilage and bone marrow regeneration was based on the isolation, expansion and implantation of cells from the patient's own tissue. Although successful in selective treatments, tissue engineering needs to overcome major challenges to allow widespread clinical application with predictable outcomes. One challenge is to present the cells in a matrix to the implantation site to allow the cells to survive th...
116 CitationsSource
Jan 1, 2005 in EMBC (International Conference of the IEEE Engineering in Medicine and Biology Society)
#1Shengju Gong (SJTU: Shanghai Jiao Tong University)H-Index: 1
#2Jian Dong (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Jin-Ye WanH-Index: 1
view all 4 authors...
A novel porous polymer scaffold fabricated by salt-leaching method for tissue engineering was developed and the mechanical properties and porosity were investigated in our study. Scanning electronic microscopy (SEM) was employed to observe the morphology and structure of porous scaffolds. Cytocompatibility of the porous scaffolds were evaluated, using HL-7702 cells as model, in terms of cell attachment and cell viabilities from 3 to 72 hours determined by MTT method. These scaffolds possessed a ...
3 CitationsSource
#1R. Adam Horch (Rice University)H-Index: 3
#2Naureen ShahidH-Index: 4
Last. Andrew R. BarronH-Index: 85
view all 6 authors...
A novel composite material has been fabricated for bone tissue engineering scaffolds utilizing the biodegradable polymer poly(propylene fumarate)/poly(propylene fumarate)-diacrylate (PPF/PPF-DA) and surface-modified carboxylate alumoxane nanoparticles. Various surface-modified nanoparticles were added to the polymer including a surfactant alumoxane, an activated alumoxane, a mixed alumoxane containing both activated and surfactant groups, and a hybrid alumoxane containing both groups within the ...
133 CitationsSource
Cited By2
Biomaterials have long been used as medical implants in humans to increase the rate of healing and prolong patient lifespan. The material of choice for the fabrication of biomaterials mimic the ECM (extracellular matrix) of humans, such as animal sourced collagen. The main source of collagen from animals are usually from bovine and porcine skin and bone wastes. However, due to local religious and cultural sensitivities in Malaysia, therefore limited research has been carried out on bovine and po...
HNV-loaded PLGA microspheres (HNV-PLGA MSs) were prepared by water-in-oil-in-water (w/o/w) double emulsion solvent evaporation technique. The surface of prepared HNV-PLGA MSs is smooth and nonporous with an average diameter of 69.9 μm. The drug-loading rate and encapsulation rate of HNV-PLGA MSs are 4.40 ± 0.26% and 48.51 ± 14.83%, respectively. Additionally, 43.36% of HNV was released from PLGA MSs after seven days of incubation. The antibacterial effects of HNV released from PLGA were as good ...
6 CitationsSource
#1Filippo Rossi (Polytechnic University of Milan)H-Index: 26
#2Marco Santoro (Rice University)H-Index: 19
Last. Giuseppe Perale (Polytechnic University of Milan)H-Index: 23
view all 3 authors...
Although bone has a high potential to regenerate itself after damage and injury, the efficacious repair of large bone defects resulting from resection, trauma or non-union fractures still requires the implantation of bone grafts. Materials science, in conjunction with biotechnology, can satisfy these needs by developing artificial bones, synthetic substitutes and organ implants. In particular, recent advances in polymer science have provided several innovations, underlying the increasing importa...
30 CitationsSource