Choleretic effects of yarrow (Achillea millefolium s.l.) in the isolated perfused rat liver.

Published on Nov 24, 2006in Phytomedicine4.268
· DOI :10.1016/J.PHYMED.2005.10.005
B. Benedek1
Estimated H-index: 1
(University of Vienna),
N. Geisz1
Estimated H-index: 1
(University of Vienna)
+ 2 AuthorsBrigitte Kopp33
Estimated H-index: 33
(University of Vienna)
Sources
Abstract
Different species from the Achillea millefolium aggregate are used against gastrointestinal and hepato-biliary disorders in traditional European medicine. In this work, a fraction enriched in dicaffeoylquinic acids (DCCAs) and luteolin-7-O-β-d-glucuronide was investigated on its choleretic effect in the isolated perfused rat liver (IPRL) compared to cynarin (1,3-DCCA), the main choleretic compound of Cynara scolymus L. A fraction containing 3,4-, 3,5- and 4,5-DCCA and luteolin-7-O-β-d-glucuronide was prepared by solid phase extraction from a 20% methanolic extract of yarrow. A total amount of 48.8% DCCAs and 3.4% luteolin-7-O-β-d-glucuronide was determined by HPLC analysis with cynarin as internal standard. IPRL experiments revealed a dose-dependant increase in bile flow (23–44–47%) by the Achillea fraction. Choleresis was two- to three-fold higher than that of cynarin. The combined effect of DCCAs and luteolin-7-O-β-d-glucuronide stimulated bile flow more effectively than the single compound cynarin. Due to their polar structure, these compounds are quantitatively extracted into teas and tinctures; hence, they seem to be the choleretic active principles in the traditional application forms of yarrow.
📖 Papers frequently viewed together
57 Citations
669 Citations
123 Citations
References8
Newest
#1Rosa Lemmens-Gruber (University of Vienna)H-Index: 20
#2Elke Marchart (University of Vienna)H-Index: 4
Last. Brigitte Kopp (University of Vienna)H-Index: 33
view all 6 authors...
The spasmolytic activity of a flavonoid fraction of a commercial sample of yarrow ( Achillea millefolium s.l.), its main flavonoids as well as quercetin and two flavonoid metabolites were investigated on isolated terminal guinea-pig ilea. The aglycones quercetin, luteolin and apigenin exhibited the highest antispasmodic activities with IC 50 values of 7.8 µmol/L, 9.8 µmol/L and 12.5 µmol/L, respectively. Rutin and the flavonoid metabolites homoprotocatechuic acid and homovanillic acid showed no ...
57 CitationsSource
#1Walter Jäger (University of Vienna)H-Index: 33
#1Walter Jaeger (University of Vienna)H-Index: 27
Last. Theresia Thalhammer (University of Vienna)H-Index: 18
view all 6 authors...
Flavopiridol (FLAP) is a novel anticancer agent that is extensively glucuronidated in patients. Biliary excretion is the main elimination pathway of FLAP conjugates responsible for enterohepatic recirculation and for the main side effect diarrhea. To investigate the hepatic transport system for FLAP glucuronides, livers of Wistar and Mrp2-deficient TR− rats were perfused with FLAP (30 μM) in a single pass system. Biliary excretion and efflux into perfusate during a 60 min period greatly differ i...
68 CitationsSource
#1Nebojsa SimicH-Index: 11
#2Radosav PalićH-Index: 21
Last. Dejan Djokovic (University of Belgrade)H-Index: 10
view all 5 authors...
Abstract The oil of a wild growing population of Achillea asplenifolia Vent, was studied for yield, composition and antibacterial activity. The major components in the oil were β-caryophyllene (17.6%), germacrene D (15.6%) and chamazulene (13.3%). In an antibacterial diffusion assay, the oil showed activity against all tested Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) and Gram-positive Staphylococcus aureus.
24 CitationsSource
#1Silvio Sosa (UniTS: University of Trieste)H-Index: 32
#1S Carrasco SosaH-Index: 30
Last. R. Della LoggiaH-Index: 22
view all 6 authors...
The topical anti-inflammatory activity of a germacrane derivative [1,4-dihydroxy-germacra-5E-10(14)-diene; DHGD] isolated from Achillea pannonica Scheele (Asteraceae) was investigated employing the Croton oil-induced dermatitis in the mouse ear. Its effects on the oedematous response and on leukocytes infiltration are described. The germacrane derivative significantly inhibited ear oedema in a dose-dependent manner, with an ID 50 of 0.40 μmol/cm 2 . DHGD (0.75 μmol/cm 2 ) provoked a global inhib...
21 CitationsSource
#1Tankred WegenerH-Index: 7
#1Wegener TH-Index: 1
Last. V. FintelmannH-Index: 1
view all 2 authors...
: The results of several clinical investigations showed the efficacy and safety of artichoke extracts (Cynara scolymus L.) in the treatment of hepato-biliary dysfunction and digestive complaints, such as sensation of fullness, loss of appetite, nausea and abdominal pain. Moreover earlier findings on a lipidlowering and hepatoprotective effect may be confirmed. In-vitro and in-vivo it has been possible to evaluate the underlying pharmacological mechanisms. Flavonoids and caffeoylquinic acids are ...
35 Citations
#1R. KirchhoffH-Index: 1
#2Ch. BeckersH-Index: 1
Last. H.J. ReimannH-Index: 1
view all 6 authors...
Abstract The choleretic action of artichoke extract [main ingredient: cynarin (1.5-di-caffeoyl-D-quinc acid)] was investigated in a randomised placebo-controlled double-blind cross-over study (pilot study) [n = 20]. The effect of the standardized, artichoke extract: Hepar SL forte (administered as a single dose: 1.92 g, by the intraduodenal route in a solution of 50 ml of water) was studied by measuring intra-duodenal bile secretion using multi-channel probes. Thirty minutes after the test-subst...
55 CitationsSource
#1U. KastnerH-Index: 2
#1U. KastnerH-Index: 8
Last. Johann JurenitschH-Index: 16
view all 7 authors...
25 CitationsSource
749 CitationsSource
Cited By83
Newest
Supercritical fluids offer considerable advantages for the isolation of natural products. Supercritical fluids can be used as antisolvents to precipitate selectively target compounds from a mixture and to remove impurities by the Supercritical Antisolvent technique (SAS). The present decade has experienced a considerable increase in the number of publications that apply this technique to natural extracts, especially for the isolation of polyphenols and carotenoids from plants and microalgae. How...
2 CitationsSource
Many Baccharis species, Asteraceae, called “carquejas” are traditional folk herbs used for the treatment of hepatic disorders in South American countries. Baccharis articulata (Lam.) Pers. and B. crispa Spreng. are included in the Argentine Pharmacopeia. However, the analysis of commercial samples showed that B. spicata (Lam.) Baill. often replaces official Baccharis spp. although there are no scientific studies that support its use. In this work, Wistar rats were administered with different dos...
Source
An herb that grows in Asia, North America and Europe, with a long history behind its clinical use. It is said to have been used since Trojan War (C. 1200 B.C.); one legend credits Chiron, the Roman Centaur, telling Achilles how to make an ointment from it to heal the bleeding wounds of the soldiers, hence the name Achillea. Dioscorides and Galen recommended its use after surgery to prevent inflammation, and to promote healing. In the Northern hemisphere, its traditional uses include digestive pr...
Source
#1Guadalupe Martínez (CONICET: National Scientific and Technical Research Council)H-Index: 5
Last. Alejandro Luis Soraci (CONICET: National Scientific and Technical Research Council)H-Index: 7
view all 9 authors...
ABSTRACTMany of the beneficial effects on productive performance observed when vegetable extracts are incorporated as feed additives in intensive farming can be explained by an increase in bile production. An experiment was conducted to study choleretic and cholagogue effect of a Cynara scolymus extract formulation and of silymarin in pigs. Pigs were cannulated with a T-tube catheter in the bile duct. Bile production was continuously measured and re-infused to the duodenum through Oddi’s sphinct...
2 CitationsSource
#1Jaruporn RakmaiH-Index: 6
#2Benjamas CheirsilpH-Index: 11
Last. Jesús Simal-GándaraH-Index: 53
view all 6 authors...
1 CitationsSource
#1Maryam Mahmoudabady (MUMS: Mashhad University of Medical Sciences)H-Index: 10
#2M. Lashkari (MUMS: Mashhad University of Medical Sciences)H-Index: 1
Last. Mohammad Soukhtanloo (MUMS: Mashhad University of Medical Sciences)H-Index: 18
view all 4 authors...
Abstract Context There are some reports about protective effects of Achillea on the heart. Objective We investigated the effect of Achillea wilhelmsii extract on cardiac function during ischemia–reperfusion (I/R) injury in the isolated rat heart. Materials and methods 60 male Wistar rats were randomly divided into 6 groups; 1: Control group, 2: Control-ischemia (CI) 3: vitamin C (10 mg/kg), 4–6: Extract groups (E 100, E 200 and E 400 mg/kg). The animals received normal saline, vitamin C or A. wi...
5 CitationsSource
#1Kota Noda (Hokkaido University)H-Index: 1
#2Eisuke Kato (Hokkaido University)H-Index: 11
Last. Jun Kawabata (Hokkaido University)H-Index: 42
view all 3 authors...
1 CitationsSource
#1Sofi Imtiyaz Ali (Annamalai University)H-Index: 3
#2B. Gopalakrishnan (Annamalai University)H-Index: 3
Last. V. Venkatesalu (Annamalai University)H-Index: 2
view all 3 authors...
Achillea millefoilum L. (Yarrow) is an important species of Asteraceae family with common utilization in traditional medicine of several cultures from Europe to Asia for the treatment of spasmodic gastrointestinal disorders, hepatobiliary, gynecological disorders, against inflammation and for wound healing. An extensive review of literature was made on A. millefoilum L. using ethno botanical text books, published articles in peer-reviewed journals, unpublished materials and scientific databases....
23 CitationsSource
#1Jaruporn Rakmai (PSU: Prince of Songkla University)H-Index: 6
#2Benjamas Cheirsilp (PSU: Prince of Songkla University)H-Index: 11
Last. Juan C. Mejuto (University of Vigo)H-Index: 28
view all 5 authors...
ABSTRACTEssential oil from yarrow (Achillea millefolium L. s. l.) has a broad spectrum of pharmacological activities. However, active chemical components of yarrow oil are sensitive to environmental factors such as, light, oxygen and temperature. Encapsulation of essential oil offers solutions for the limitation. Yarrow oil was encapsulated in hydroxypropyl-β-cyclodextrin (HPβCD) through freeze-drying technique with encapsulation efficiency of 45%. Inclusion complex formation was examined by sca...
35 CitationsSource
#1David Villanueva-Bermejo (CSIC: Spanish National Research Council)H-Index: 5
#2Fouad Zahran (Helwan University)H-Index: 10
Last. Tiziana Fornari (CSIC: Spanish National Research Council)H-Index: 27
view all 6 authors...
Abstract In this work the fractionation of an ethanolic extract of Achillea millefolium L. (yarrow) using supercritical carbon dioxide anti-solvent technique was studied, with the target of increasing the concentration of phenolic compounds in the precipitated fraction. The effect of pressure on the selective precipitation was analyzed, together with the morphology and particle size distribution of the precipitates. In the range of pressures studied (10–20 MPa), up to a 3 fold increase of the to...
16 CitationsSource