Simultaneous determination of imperatorin and its metabolite xanthotoxol in rat plasma by using HPLC–ESI-MS coupled with hollow fiber liquid phase microextraction

The objective of the present study was to develop a new method for the simultaneous quantitation of imperatorin and its metabolite xanthotoxol in rat plasma. The samples were prepared with hollow fiber liquid phase microextraction (HF-LPME). The optimized extraction procedure was acquired by assessing extraction solvent, length of the fiber, agitation rate, extraction temperature and time. A comparison of sample pretreatment ways between HF-LPME...