1H-NMR metabonomics study of the therapeutic mechanism of total alkaloids and ajmalicine from Rauvolfia verticillata in spontaneously hypertensive rats
The total alkaloids extracted from Rauvolfia verticillata have been developed into drugs-Verticil to treat hypertension. It has a slow and sustained reduction in blood pressure, for the treatment of early and mild hypertension. Ajmalicine, an indole alkaloid from total alkaloids, has been shown to not only reduce blood pressure but also expand blood vessels. However, the mechanism of the antihypertensive effect of total alkaloids and ajmalicine has not been fully characterized. In this research, we chose to study a hypertensive rodent model, spontaneously hypertensive rats. Spontaneously hypertensive rats are a useful model for the study of essential hypertension which is a complex, polygenic, and multifactorial disorder. Studies suggest the usefulness of an 1H-NMR-based metabonomic approach using spontaneously hypertensive rats for the study of hypertension. The spontaneously hypertensive rats were given total alkaloids and ajmalicine by oral gavage from 10 to 16 weeks of age. Plasma samples were analyzed using 1H-NMR spectroscopy and metabolic information was extracted from the NMR data by principal components analysis as well as partial least square-discrimination analysis in order to develop biomarkers associated with hypertension. The metabolic pathways of small molecule metabolites in plasma were investigated to explain the content changes. The results obtained showed that ajmalicine only has a weak effect for hypertension. Total alkaloids exert their antihypertensive effect by reducing the level of tyrosine and increasing the level of choline and leucine. The use of total alkaloids plus ajmalicine showed its antihypertensive effect more strongly than total alkaloids.
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Gynostemma pentaphyllum (GP) is widely used for the treatment of diseases such as hyperlipidemia, fatty liver and obesity in China, and atorvastatin is broadly used as an anti-hyperlipidemia drug. This research focuses on the plasma and liver metabolites in the following four groups of rats: control, a hyperlipidemia model, a hyperlipidemia model treated with GP and a hyperlipidemia model treated with atorvastatin. Using 1H-NMR-based metabonomics, we elucidated the therapeutic mechanisms of GP a...
Polycystic ovary syndrome (PCOS) is a common, clinically heterogeneous endocrine disorder affecting women of reproductive age, associated with endocrinopathy and metabolic abnormalities. Although some metabolic parameters have been investigated, very little information has been reported on the changes of small metabolites in biofluids. The aim of this study was to establish the metabolic profile of PCOS and compare it with that of controls. In this cross-sectional study of 34 women with PCOS and...
Metabolomics first burst into the toxicology arena approximately 10–12 years ago, experiencing rapid growth as a science in the intervening period (Fig. 1). Though metabolomics still trails the publication rates of its sister technologies, genomics and proteomics, it is closing the gap in terms of both the numbers of publications and the quality of those publications. One continuing source of confusion is differentiating the terms metabolomics, metabonomics, and metabolic profiling. Although met...
A UPLC/TOF-MS-based metabonomic study was conducted to assess the holistic efficacy of Traditional Chinese Medicine Shuanglong Formula (SLF) for myocardial infarction in rats. Thirty male Sprague−Dawley rats were randomly divided into five groups after surgery. The Panax ginseng group, Salvia miltiorrhiza group, and SLF group were treated with water extractions of Panax ginseng (PG), Salvia miltiorrhiza (SM), and SLF (the ratio of SM to PG was 3:7) at a dose of 5 g/kg·w·d for 21 consecutive days...
Last. Anguo Zhou(Sichuan Agricultural University)H-Index: 1
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This study investigates the effect of acute cysteamine (CS) supplementation on rat metabolism. A metabolomic strategy using high-resolution 1H-NMR spectroscopy in conjunction with principal component analysis was applied to examine rat biological responses to CS administration. Half of female Sprague–Dawley rats (2 groups of 6 rats) were each given doses of 150 mg CS/kg body weight intraperitoneally. Urine samples were collected twice daily (0–8 and 8–24 h) from the rats following CS administrat...
Significant improvements in NMR technology and methods have propelled NMR studies to play an important role in a rapidly expanding number of applications involving the profiling of metabolites in biofluids. This review discusses recent technical advances in NMR spectroscopy based metabolite profiling methods, data processing and analysis over the last three years.
Abstract Urinary metabolic perturbations associated with liver toxicity induced by Huang-yao-zi (root of Dioscorea bulifera L.) were studied using nuclear magnetic resonance spectroscopy ( 1 H NMR) to determine the correlations between metabonomic profiling and histopathologic/biochemical observations and to discover biomarkers for liver toxicity. Huang-yao-zi with a maximal tolerance dose (MTD) was given to male Sprague–Dawley rats for 72 h followed by metabonomic analysis of urine samples coll...
#1Yong-Min Lao(SCUT: South China University of Technology)H-Index: 7
#2Jian-Guo Jiang(SCUT: South China University of Technology)H-Index: 34
Last. Lu Yan(SCUT: South China University of Technology)H-Index: 1
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In the recent years, a wide range of metabonomic analytical techniques are widely used in the modern research of traditional Chinese medicine (TCM). At the same time, the international community has attached increasing importance to TCM toxicity problems. Thus, many studies have been implemented to investigate the toxicity mechanisms of TCM. Among these studies, many metabonomic-based methods have been implemented to facilitate TCM toxicity investigation. At present, the most prevailing methods ...
Soy sauce, a well-known seasoning in Asia and throughout the world, consists of many metabolites that are produced during fermentation or aging and that have various health benefits. However, their comprehensive assessment has been limited due to targeted or instrumentally specific analysis. This paper presents for the first time a metabolic characterization of soy sauce, especially that aged up to 12 years, to obtain a global understanding of the metabolic variations through 1H NMR spectroscopy...
Organisms often respond in complex and unpredictable ways to stimuli that cause disease or injury. By measuring and mathematically modelling changes in the levels of products of metabolism found in biological fluids and tissues, metabonomics offers fresh insight into the effects of diet, drugs and disease.
Last. Shu-Qi Wang(SDU: Shandong University)H-Index: 12
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Rauvolfia vomitoria is widely distributed in the tropical regions of Africa and Asia, and has been used in traditional folk medicine in China. Indole alkaloids were found to be major bioactive components, while the effects of diabetes mellitus on the pharmacokinetic parameters of the components have not been reflected in vivo. In this study, an efficient and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of five ingr...
: Semen descurainiae oil (SDO) is an important traditional Chinese medicine that was recently discovered to have the function of reducing blood lipids. Metabolomics analyses of plasma, liver and kidney in rats were performed using 1 H-NMR and LC-MS to illuminate the lower blood lipid concentration effect of SDO, and niacin was considered as the active control. The measure of total cholesterol (TC) and low-density-lipoprotein cholesterol (LDL-C) in plasma showed that SDO treatment decreased signi...
Last. Longshan Zhao(HHIT: Huaihai Institute of Technology)H-Index: 1
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Abstract Guizhi Fuling capsule (GFC) was an important traditional Chinese herbal medicine used for the treatment of primary dysmenorrheal (PD). The aim of this study was to evaluate the anti-dysmenorrheal effect of GFC on dysmenorrheal rats induced by oxytocin and to investigate its mechanism of action. An integrative urinary metabolomic study based on 1H NMR and UPLC–MS was used to investigate the therapeutic effect of GFC on PD rats. In addition, in order to obtain more potential biomarkers an...
#1Sunil Kumar(Central Drug Research Institute)H-Index: 15
#1Sunil Kumar(Central Drug Research Institute)H-Index: 24
Last. Mohan Lal(North East Institute of Science and Technology)H-Index: 14
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Abstract A rapid, sensitive and reproducible method using ultra-high-performance liquid chromatography coupled with electrospray ionization hybrid triple quadrupole-linear ion trap mass spectrometry (UHPLC-ESI-QqQ LIT -MS/MS) in multiple reaction monitoring (MRM) mode was developed and validated for simultaneous quantitation of anticancer (vincristine, vinblastine, vindesine), antihypertensive (ajmaline, ajmalicine, reserpine), aphrodisiac (yohimbine), sedative (serpentine) agents, dietary suppl...
#1Sunil Kumar(Central Drug Research Institute)H-Index: 15
#1Sachin Kumar(Central Drug Research Institute)H-Index: 14
Last. Brijesh Kumar(Central Drug Research Institute)H-Index: 10
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RATIONALE: Catharanthus roseus is a well-known dicotyledonous medicinal plant containing diverse classes of bioactive terpene indole alkaloids (TIAs), in particular the anticancer agents vinblastine and vincristine. In view of the commercial importance of these compounds there is an urgent need to develop an accurate and reliable method for the screening of TIAs from C. roseus. METHODS: A method for the separation and characterization of these compounds was developed using high-performance liqui...
Last. Yue-Hu Pei(SPU: Shenyang Pharmaceutical University)H-Index: 25
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Objectives Baicalin is the main bioactive flavonoid constituent isolated from Scutellaria baicalensis Georgi. The mechanisms of protection of liver remain unclear. In this study, 1H NMR-based metabonomics approach has been used to investigate the alleviation effect of Baicalin. Method 1H NMR metabolomics analyses of urine and serum from rats, was performed to illuminate the alleviation effect of Baicalin on mineral medicine (cinnabar)-induced liver and kidney toxicity. Results The metabolic prof...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Abstract Ethnopharmacological relevance Cortex Fraxini (CF) is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. Aim of the study The aim of this study was to evaluate the anti-hyperuricemic effect of CF on hyperuricemic rats and to investigate its mechanism of action. Materials and methods Metabolomics based on NMR and MS was used to study the therapeutic effect of CF on hyperuricemic rats. Plasma determination of uric acid (UA) showed that CF tr...