Supercritical fluid extraction of isoflavones from biological samples with ultra‐fast high‐performance liquid chromatography/mass spectrometry

An efficient method of modifier addition for supercritical fluid extraction (SFE) of polar isoflavones was developed and yielded extraordinarily high recoveries. To find the optimal extraction conditions, a temperature and pressure optimization and modifier impact study was performed in naturally contaminated and spiked samples. Ultra‐fast high‐performance liquid chromatography/mass spectrometry (HPLC/MS) was used for the determination of...