Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro.

Published on Jan 15, 2016in General and Comparative Endocrinology2.822
· DOI :10.1016/J.YGCEN.2015.04.001
Xinwei Li34
Estimated H-index: 34
(JLU: Jilin University),
Yuan Guan8
Estimated H-index: 8
(JLU: Jilin University)
+ 6 AuthorsGuowen Liu27
Estimated H-index: 27
(JLU: Jilin University)
Abstract Fatty liver is a major metabolic disorder of dairy cows. One important reason is that hepatic very low-density lipoproteins (VLDL) assembly was significant decreased in dairy cows with fatty liver. In addition, the impairment of insulin-like growth factor (IGF)-1 synthesis was involved in the development of fatty liver. Therefore, the objective of this study was to investigate the effects of IGF-1 on the VLDL assembly in cow hepatocytes. In this study, cow hepatocytes were cultured and then transfected with Ad-GFP-IGF-1 (inhibited the IGF-1 expression) and Ad-GFP (negative control), and treated with different concentrations of IGF-1, respectively. The results showed that IGF-1 increased the mRNA abundance of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), microsomal triglyceride transfer protein (MTTP), and low-density lipoprotein receptor (LDLR) and then increased the VLDL assembly in cow hepatocytes. Nevertheless, impairment of IGF-1 expression by Ad-GFP-IGF-1 could inhibit above genes expression and VLDL assembly in hepatocytes. Taken together, these results indicate that IGF-1 increases the VLDL assembly and impairment of IGF-1 expression decreases the VLDL assembly in cow hepatocytes.
Abstract Non-esterified fatty acids (NEFAs) are important induction factors of inflammatory responses in some metabolic diseases. High plasma levels of NEFAs and oxidative stress exist in the dairy cows with ketosis. The aim of this study was to investigate whether high levels of NEFAs can induce inflammatory response and the specific molecular mechanism in the hepatocytes of dairy cow. In vitro , primary cultured bovine hepatocytes were treated with different concentrations of NEFAs, PDTC (an N...
Abstract The natural incidence of fatty liver in ruminants is significantly higher than in monogastric animals. Fatty liver is associated with sterol regulatory element-binding protein 1c (SREBP-1c). The aim of this study was to investigate the regulatory network effects of SREBP-1c on the lipid metabolic genes involved in fatty acid uptake, activation, oxidation, synthesis, and very low-density lipoprotein (VLDL) assembly in bovine hepatocytes. In vitro , bovine hepatocytes were transfected wit...
#8Liancheng Lei (JLU: Jilin University)H-Index: 22
Abstract High serum concentrations of nonesterified fatty acids (NEFA), which may affect the synthesis and assembly of very low density lipoproteins (VLDL), are associated with fatty liver during the early lactation period. Therefore, the objective of this study was to investigate the effects of NEFA on the synthesis and assembly of VLDL in bovine hepatocytes. Bovine hepatocytes were cultured and treated with different concentrations of NEFA. The mRNA expression of apolipoprotein B100 ( ApoB100 ...
#1Zhigang ZhangH-Index: 25
#2Xiaobing Li (JLU: Jilin University)H-Index: 23
Last. Ri-He Zhang (JLU: Jilin University)H-Index: 1
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Abstract Primary hepatocytes are commonly used during in vitro studies, but care must be taken with isolation and culture of the cells to ensure their viability. In this study, hepatocytes were isolated from the liver (caudate process) of a newborn calf by the collagenase perfusion and digestion method. The trypan blue exclusion method was used to determine total cell number and the survival rate of hepatocytes, while hepatocyte function was assessed by measuring lactate dehydrogenase, albumin a...
#1Winnie Luu (UNSW: University of New South Wales)H-Index: 13
#2Laura J. Sharpe (UNSW: University of New South Wales)H-Index: 23
Last. Andrew J. Brown (UNSW: University of New South Wales)H-Index: 60
view all 4 authors...
Akt is an essential protein kinase for cell growth, proliferation, and survival. Perturbed Akt regulation is associated with a number of human diseases, such as cancer and diabetes. Recently, evidence has emerged that Akt is involved in the regulation of the sterol-regulatory element binding proteins, which are master transcriptional regulators of lipid metabolism. This offers a means by which synthesis of new membrane can be coordinated with cell growth and proliferation. However, the link betw...
#1H.A. van Dorland (University of Bern)H-Index: 17
#2Hassan Sadri (University of Tabriz)H-Index: 12
Last. Rupert M. Bruckmaier (University of Bern)H-Index: 61
view all 4 authors...
Dairy cows with high and low plasma non-esterified fatty acid (NEFA) concentrations in early lactation were compared for plasma parameters and mRNA expression of genes in liver and subcutaneous adipose tissue. The study involved 16 multiparous dairy cows with a plasma NEFA concentration of >500 mumol/l [n = 8, high NEFA (HNEFA)] and <140 mumol/l [n = 8, low NEFA (LNEFA)] in the first week post-partum (pp). Blood samples, adipose and liver tissues were collected on day 1 (+1d) and at week 3 pp (+...
#1A. StarkeH-Index: 8
#2S. SchmidtH-Index: 1
Last. Jürgen RehageH-Index: 24
view all 7 authors...
Abstract The objective of the study was to investigate portal blood flow (PBF) in dairy cows with fatty liver by means of Doppler ultrasonography. Eighty lactating German Holstein cows less than 100 d in milk were used (mean ± standard error of the mean; body weight: 583±9kg, age: 5±0.2 yr, withers height: 145.4±0.5cm, milk yield: 9±0.6kg). All cows had left abomasal displacement and underwent omentopexy via right flank laparotomy. The size of the liver and the thickness over the portal vein wer...
#1Johanna L. Barclay (UQ: University of Queensland)H-Index: 20
#2Caroline N. Nelson (UQ: University of Queensland)H-Index: 5
Last. Michael J. Waters (UQ: University of Queensland)H-Index: 73
view all 8 authors...
GH deficiency is known to be clinically associated with a high incidence of nonalcoholic fatty liver disease, and this can be reversed by GH administration. Here we investigated the mechanistic basis for this phenomenon using engineered male mice lacking different signaling elements of the GH receptor, hepatic stat5a/b−/− mice and a mouse hepatoma line. We found deficient GH-dependent signal transducer and activator of transcription (STAT)-5 signaling correlates with steatosis, and through micro...
#1Archana Vijayakumar (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 19
#2Ruslan Novosyadlyy (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 15
Last. Derek LeRoith (ISMMS: Icahn School of Medicine at Mount Sinai)H-Index: 124
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This review will summarize the metabolic effects of growth hormone (GH) on the adipose tissue, liver, and skeletal muscle with focus on lipid and carbohydrate metabolism. The metabolic effects of GH predominantly involve the stimulation of lipolysis in the adipose tissue resulting in an increased flux of free fatty acids (FFAs) into the circulation. In the muscle and liver, GH stimulates triglyceride (TG) uptake, by enhancing lipoprotein lipase (LPL) expression, and its subsequent storage. The e...
#1Yong Fan (University of Pittsburgh)H-Index: 16
#2Ram K. MenonH-Index: 21
Last. Mark A. Sperling (University of Pittsburgh)H-Index: 42
view all 10 authors...
Growth hormone (GH) plays a pivotal role in growth and metabolism, with growth promotion mostly attributed to generation of insulin-like growth factor I (IGF-I) in liver or at local sites of GH action, whereas the metabolic effects of GH are considered to be intrinsic to GH itself. To distinguish the effects of GH from those of IGF-I, we developed a Cre-lox-mediated model of tissue-specific deletion of the growth hormone receptor (GHR). Near total deletion of the GHR in liver (GHRLD) had no effe...
Cited By1
#1Arash Shahsavari (UQ: University of Queensland)H-Index: 2
#2Michael J. D'Occhio (USYD: University of Sydney)H-Index: 27
Last. Rafat Al Jassim (UQ: University of Queensland)H-Index: 14
view all 3 authors...
High-producing dairy cows enter a period of negative energy balance during the first weeks of lactation. Energy intake is usually sufficient to cover the increase in energy requirements for fetal growth during the period before calving, but meeting the demand for energy is often difficult during the early stages of lactation. A catabolic state predominates during the transition period, leading to the mobilisation of energy reserves (NEFA and amino acids) that are utilised mainly by the liver and...
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