Organotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration
Abstract
Background aims The aim of the present study was to evaluate the effects of air-liquid interface on the differentiation potential of human amnion epithelial cells (HAECs) to skin-like substitute in organotypic culture. Methods HAECs at passage 1–2 were seeded onto a fibrin layer populated with human amnion mesenchymal cells to form the organotypic cultures. The organotypic HAECs were then cultured for 7, 14 and 21 d in two types of culture...
Paper Details
Title
Organotypic culture of human amnion cells in air-liquid interface as a potential substitute for skin regeneration
Published Date
Aug 1, 2013
Journal
Volume
15
Issue
8
Pages
1030 - 1041
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