Application of an LC–UV method to estimate lutein recovery during infant formula manufacture

Lutein is a carotenoid that is considered to be important to the integrity of the retina and is therefore increasingly being supplemented into bovine milk-based paediatric formulae to levels equivalent to those found in human milk. A simple analytical method has been developed and intra-laboratory validated to facilitate routine in-process control of lutein addition. The method involves dilution of a carotenoid premix, followed by C30...