Abstract The aim of the present work was the development of a powder formulation for the combined delivery of platelet lysate and of a model antibiotic drug, vancomycin hydrochloride (VCM), in chronic skin ulcers. Calcium alginate particles were prepared by freeze-drying beads obtained by ionic gelation method. The experimental conditions adopted permitted the complete loading of VCM and of PDGF AB, the growth factor chosen as representative of those contained in PL. Such particles where able to absorb PBS (mimicking wound exudate), to form a gel and to modulate the release of VCM and of PDGF AB. They are characterized by enhancement properties of human fibroblast proliferation due to PL presence. In particular, PL, when loaded in alginate particles, was able not only to increase the number of viable cells, but also the number of cells in proliferative phase. Such properties were comparable to those of fresh PL indicating the capability of calcium alginate particles to load PL bioactive substances without altering their activity. The formulation developed is characterized by an easier and a less painful administration with respect to traditional gauzes and semisolid preparations and permits the loading in the same dosage form of active substances of different nature avoiding eventual incompatibility problems.
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 7 authors...
AbstractContext: The local treatment of vaginal mucositis requires an intimate and prolonged contact of anti-infective drugs with the mucosa. This can be achieved by means of mucoadhesive and thermally sensitive vehicles, capable of gelifying at the physiological temperature.Objective: The aim of the present work was to compare the potentiality of poloxamer 407 (PLX)/chitosan lactate (CS-L) and CS-L/glycerophosphate (GP) mixtures as mucoadhesive thermally sensitive vehicles for the treatment of ...
There are still so-called unmet needs in the treatment of epithelial and cutaneous lesions. Mucositis, ocular lesions, chronic skin wounds represent typical examples. These pathologies do not yet afford a satisfactory treatment. In particular chronic wounds represent a major health care burden, likely to increase as the population ages. Healing of epithelial and cutaneous lesions progresses through a complex cascade of events starting with the secretion in the local environment of a pool of grow...
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 8 authors...
The aim of the present work was the development of sponge-like dressings, obtained by freeze-drying, based on chitosan glutamate and sodium hyaluronate for platelet lysate (PL) delivery to chronic skin wounds. A first phase of the research focused on the choice of the best dressing composition to obtain formulations endowed with the desired mechanical and hydration properties. In particular glycine amount (cryoprotectant agent), and water content were considered as formulation variables. The add...
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 8 authors...
Abstract Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact of platelet lysate (PL) with cornea ulcers. A sterile vehicle based on chondr...
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 8 authors...
Oral mucositis is an inflammatory disease of the mucosa lining oral cavity which leads to atrophy of the epithelium and to its ulceration to form chronic lesions. Many studies, both in vitro and in vivo, have disclosed the effectiveness of growth factors derived from platelets to enhance cell proliferation, differentiation, chemotaxis, angiogenesis and extracellular matrix synthesis involved in the healing of tissues. Despite potential usefulness of growth factors, animalderived or genetically e...
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 8 authors...
Optimal treatment of oral mucositis (OM) due to graft versus host disease (GvHD) is currently not available. Platelet-derived growth factors (PDGFs) have high capability for tissue healing and may play a role in repairing the mucosal barrier. The aim of the present work was to develop a mucoadhesive formulation to administer platelet lysate to oral cavity prolonging contact time of platelet lysate with oral mucosa. The mucoadhesive formulation was characterized for in vitro properties (PDGF-AB c...
Last. Carla Caramella(UNIPV: University of Pavia)H-Index: 57
view all 9 authors...
Objectives Growth factors contained in platelet α-granules initiate and modulate tissue repair and are proposed for the treatment of soft and hard-tissue surgical conditions and in the management of non-healing wounds. Platelet lysate is a hemoderivative obtained from platelet-rich plasma and is capable of releasing a pool of growth factors. Many medical and surgical techniques have been proposed for the treatment of corneal lesions; management of these conditions remains problematic and healing...
The topical use of platelet concentrates is recent and its efficiency remains controversial. Several techniques for platelet concentrates are available; however, their applications have been confusing because each method leads to a different product with different biology and potential uses. Here, we present classification of the different platelet concentrates into four categories, depending on their leucocyte and fibrin content: pure platelet-rich plasma (P-PRP), such as cell separator PRP, Vi...
Summary Background Platelets play a pivotal role in wound healing. Their beneficial effect is attributed to the release of bioactive substances, although the involved mechanisms are mostly unknown. Objectives To investigate mechanisms underlying platelet-induced wound healing using HaCaT keratinocytes, representing an in vitro model of proliferating and migrating keratinocytes. Methods Cells were exposed to platelet lysate (PL) purified from whole blood samples. Cell metabolism and proliferation...
Last. Viness Pillay(University of the Witwatersrand)H-Index: 48
view all 5 authors...
Recent research has been successful in demonstrating the importance of the addition of platelets to the field of cell-mediated therapeutics, by making use of different platelet forms to design modalities able to positively impact a wide range of diseases. A key obstacle hindering the success of conventional therapeutic interventions is their inability to produce targeted treatment, resulting in a number of systemic side effects and a longer duration for the onset of action to occur. An additiona...
Introduction: Years of tissue engineering research have clearly demonstrated the potential of integrating growth factors (GFs) into scaffolds for tissue regeneration, a concept that has recently be...
Abstract Platelet lysate (PL), a blood product that contains high concentrations of growth factors (GFs), can be considered as a cost-effective source of multiple GFs. In this study, hyaluronic acid (HA) based microgels were developed for delivery of PL proteins. Spherical microgel were prepared using a water in oil emulsion method. First, hyaluronic acid was grafted with tyramine groups, after which prepared microdroplets were crosslinked via an enzymatic reaction in the presence of hydrogen pe...
Due to their outstanding properties in terms of mild gelation conditions and simple functionalization, biocompatibility, low toxicity, biodegradability, non-antigenicity and chelating ability, as well as relatively low cost, alginates have been widely used in a variety of biomedical applications including tissue engineering and drug delivery systems. Smart alginate hydrogels for on-demand drug release in response to environmental stimuli and 3D bioprinting will play an important role in the futu...
#2Silvia Rossi(UNIPV: University of Pavia)H-Index: 38
Last. Franca Ferrari(UNIPV: University of Pavia)H-Index: 46
view all 9 authors...
: Chronic wounds are the result of alterations in the complex series of events of physiological wound healing. In particular, the prolonged inflammation results in increased protease activity, in the degradation of extracellular matrix (ECM) and of growth factors (GFs). The relevance of platelet GFs in maintaining and restoring the complex equilibrium of different moments in wound healing is well recognized. Moreover, the observed decrease of their levels in chronic wounds suggested a possible t...
#1Silvia Rossi(UNIPV: University of Pavia)H-Index: 38
#2Michela Mori(UNIPV: University of Pavia)H-Index: 17
Last. Franca Ferrari(UNIPV: University of Pavia)H-Index: 46
view all 8 authors...
Abstract The aim of the present work was to develop a medication allowing for the combined delivery of platelet lysate (PL) and an anti-infective model drug, vancomycin hydrochloride (VCM), to chronic skin ulcers. A simple method was set up for the preparation of hyaluronic acid (HA) core-shell particles, loaded with PL and coated with calcium alginate, embedded in a VCM containing alginate matrix. Two different CaCl2 concentrations were investigated to allow for HA/PL core-shell particle format...
#1Era Jain(SLU: Saint Louis University)H-Index: 15
#2Saahil Sheth(SLU: Saint Louis University)H-Index: 5
Last. Scott A. Sell(SLU: Saint Louis University)H-Index: 28
view all 5 authors...
Platelet-rich plasma (PRP), an autologous blood derived product is a concentrated mix of multiple growth factors and cytokines. Direct injections of PRP are clinically used for treatment of various musculoskeletal disorders and in wound healing. However, PRP therapy has met with limited clinical success possibly due to unpredictable and premature bolus delivery of PRP growth factors. The objective of this study was to predictably control the bioavailability of PRP growth factors using a hydrolyt...
#1Marika Tenci(UNIPV: University of Pavia)H-Index: 6
#2Silvia Rossi(UNIPV: University of Pavia)H-Index: 38
Last. Franca Ferrari(UNIPV: University of Pavia)H-Index: 46
view all 10 authors...
Abstract The aim of the present work was the development of polymer films loaded with a carvacrol (CVR)/clay hybrid (HYBD) for the delivery of CRV in infected skin ulcer treatment. Different clays were considered: montmorrilonite, halloysite and palygorskite (PHC). CRV incorporation in PHC reduced its volatility. HYBD showed 20% w/w CRV loading capacity and was able to preserve CRV antioxidant properties. HYBD was characterized by improved antimicrobial properties against S. aureus and E. coli a...
#1Wing Y. Ng(Albany College of Pharmacy and Health Sciences)H-Index: 1
#2Amanda Migotto(USP: University of São Paulo)H-Index: 4
Last. Luciana B. Lopes(Albany College of Pharmacy and Health Sciences)H-Index: 29
view all 4 authors...
Abstract Alginate beads containing the polar lipid monoolein were developed as a strategy to manage wet wounds by providing improved uptake of excess exudate while releasing adenosine locally for promotion of healing. To obtain monoolein-containing beads, the lipid was mixed with almond oil (2:1 w/w), and emulsified within the alginate aqueous dispersion, followed by ionotropic gelation in CaCl 2 solution. Compared to alginate-only, monoolein-alginate systems were 1.44-fold larger, their swellin...
Last. Livia Visai(UNIPV: University of Pavia)H-Index: 59
view all 13 authors...
The synthesis of Ag nanoparticles from Ag+ has been investigated, with pectin acting both as reductant and coating. ∼100% Ag+ to Ag(0) one-pot conversion was obtained, yielding p-AgNP, i.e. an aqueous solution of pectin-coated spherical Ag nanoparticles (d = 8.0 ± 2.6 nm), with a < 1 ppm concentration of free Ag+ cation. Despite the low free Ag+ concentration and low Ag+ release with time, the nature of the coating allows p-AgNP to exert excellent antibacterial and antibiofilm actions, comparabl...