Size-exclusion chromatography in an analytical perspective

Modern media for size-exclusion chromatography combine maximum selectivity with high plate count to resolve solutes differing in molecular mass by as little as 20%. Separations of solutes differing in molecular mass by only 10% will be very hard to accomplish even for rod-shaped molecules. The reduced column lengths and porosity of small-particle-sized media unfortunately reduces the inherent resolving power to the same order as traditional...