Griess micro-assay for the determination of nitrite by combining fibre optics-based cuvetteless UV–Vis micro-spectrophotometry with liquid-phase microextraction

A novel, simple and low cost method for the determination of nitrite using headspace single-drop microextraction and cuvetteless ultraviolet–visible micro-spectrophotometry is described. A Griess reagent-containing aqueous microdrop exposed to the headspace was used as extractant of the volatile nitrogen oxides generated from nitrite by direct acidification of the aqueous sample. Experimental parameters affecting the headspace single-drop...