Analytica Chimica Acta
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#1Luyao LiuH-Index: 3
Last. Feimeng ZhouH-Index: 56
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Abstract The tris-nitrilotriacetic acid (tris-NTA) chip has been used for surface plasmon resonance (SPR) kinetic studies involving histidine (His)-tagged proteins. However, its full potential, especially for analyte quantification in complex biological media, has not been realized due to a lack of systematic studies on the factors governing ligand immobilization, surface regeneration, and data analysis. We demonstrate that the tris-NTA chip not only retains His-tagged proteins more strongly tha...
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#1Cheol-Kyun Choi (SKKU: Sungkyunkwan University)
#2Samy M. Shaban (SKKU: Sungkyunkwan University)H-Index: 27
Last. Dong-Hwan Kim (SKKU: Sungkyunkwan University)H-Index: 40
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Abstract Smartphone-assisted point-of-care (POC) bioassay has brought a giant leap in personal healthcare system and environmental monitoring advancements. In this study, we developed a rapid and reliable colorimetric urea biosensor assisted by a smartphone. We employed hydrolysis of urea into NH3 by urease, which activates the reduction power of tannic acid, to generate silver nanoparticles for a dramatic colorimetric response. The proposed urea biosensor was validated in a solution to provide ...
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#1Ángela Écija-Arenas (UCO: University of Córdoba (Spain))H-Index: 2
#2Eva-Maria Kirchner (University of Regensburg)H-Index: 3
Last. Juan Manuel Fernández-Romero (UCO: University of Córdoba (Spain))H-Index: 16
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Abstract A biosensor in which an affinity reaction occurs in the sensitive microzone through the use of specific aptamers to determine kanamycin residues in agri-food samples has been developed. It is an irreversible and continuous flow aptameric biosensor (aptasensor) in which the signal variations are monitored by surface plasmon resonance (SPR) measurements based on the specific interaction of the aptamer with the antibiotic. The signal variation is proportional to the analyte concentration. ...
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Abstract Nucleotide-binding proteins play important roles in a variety of biological processes. While ATP- and GTP-binding proteins have been well studied, the systematical identification of UTP-interacting proteins remains under investigated. Here, we developed a chemical proteomic strategy using a biotinylated UTP affinity probe coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) method to enrich, identify and quantify UTP-binding proteins at the entire proteome scale. By pe...
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#1Weifeng Zhang (Beihang University)
#2Yun Li (PKU: Peking University)
Last. Weili Hong (Beihang University)
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Abstract Methods for rapid antimicrobial susceptibility testing (AST) are urgently needed to address the emergence and spread of antimicrobial resistance. Here, we report a new method based on stimulated Raman scattering (SRS) microscopy, which measures both the metabolic activity and the morphological deformation of bacteria to determine the antimicrobial susceptibility of β-lactam antibiotics rapidly. In this approach, we quantify single bacteria's metabolic activity by the carbon-deuterium (C...
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#1Vipul Gupta (UTAS: University of Tasmania)H-Index: 9
#2Brett Paull (UTAS: University of Tasmania)H-Index: 53
Abstract Microfluidic distributors that can uniformly distribute fluid from a single channel to multiple channels and into, or across, 3D spaces and vice versa has always represented a challenge. Recently, significant interest has been observed in 3D printing three-dimensional flow distributors. However, they either lack their use at low flow rates or in high aspect ratio environments, which are usually encountered in various applications, such as generating organs-on-a-chip, chromatographic col...
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#1Yuexia YangH-Index: 1
#2Liang ZhangH-Index: 2
Last. Yun LiuH-Index: 2
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Abstract Leucine aminopeptidase (LAP) as an important proteolytic enzyme, has been mainly found in hepatobiliary cells, and overexpressed in hepatoma cells. Herein, a new highly selective red-emitting fluorescent probe (DCDHF-Ala) for LAP has been synthesized based on 2-dicyanomethyldiene-3-cyano-2, 5-dihydrofuran (DCDHF) as fluorophore, and alanine (Ala) as the detection group. More importantly, it's the first time to use Ala as a reactive group for LAP. DCDHF-Ala has a low detection limit (0.2...
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Abstract Androgenic anabolic steroids are the most misused substances in sports because of their performance-enhancing effects. Often synthetic analogues of endogenously present steroids are administered. To determine their endogenous or exogenous origin, Gas Chromatography Combustion Isotope Ratio Mass Spectrometry (GC-C-IRMS) is used in the field of doping control. Compounds subjected to IRMS analysis must be interference-free, with liquid chromatography fraction collection (HPLC-FC) being the...
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#1Lunjie Huang (SCUT: South China University of Technology)H-Index: 21
#2Da-Wen SunH-Index: 133
Last. Qingyi Wei (SCUT: South China University of Technology)H-Index: 21
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Abstract Efficient identification of pathogenic bacteria is greatly concerned with microbial food safety and foodborne diseases diagnosis. Surface-enhanced Raman scattering (SERS) tags are among the cutting-edge tools for bioanalysis, but facing troubles in either SERS sensitivity, durability, interfering signals, or universal recognition agents for target bacteria. This work proposed a multivariate scheme enabled by polyphenolic chemistry for the green synthesis, facile stabilization (functiona...
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#1Nguyen Ngoc VietH-Index: 1
#2Le Viet ThongH-Index: 2
Last. Nguyen Van HieuH-Index: 40
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Abstract The effect of MoS2 nanosheet (NS) decoration on the gas-sensing properties of SnO2 nanofibers (NFs) was investigated. The decorated sensors were fabricated by facile on-chip electrospinning technique and subsequently dropping MoS2 NSs-dispersed solution. The MoS2 NS decoration resulted in enhanced the response and reduced the operating temperature of SnO2 NFs towards SO2 gas. The SnO2 NF sensor decorated with the optimum density of MoS2 NSs exhibited about 10-fold enhancement in gas res...
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Top fields of study
Analytical chemistry
Detection limit
Inorganic chemistry
Chemistry
Chromatography