Gradient retention prediction of acid–base analytes in reversed phase liquid chromatography: A simplified approach for acetonitrile–water mobile phases
Abstract
In previous work, a two-parameter model to predict chromatographic retention of ionizable analytes in gradient mode was proposed. However, the procedure required some previous experimental work to get a suitable description of the pKa change with the mobile phase composition. In the present study this previous experimental work has been simplified. The analyte pKa values have been calculated through equations whose coefficients vary depending on...
Paper Details
Title
Gradient retention prediction of acid–base analytes in reversed phase liquid chromatography: A simplified approach for acetonitrile–water mobile phases
Published Date
Nov 1, 2014
Journal
Volume
1370
Pages
129 - 134
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