Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC-MS(n) combined with bioassays.
Abstract Fufang Ejiao Syrup (FES) is a widely used immune-boosting Traditional Chinese Medicine (TCM) in Eastern Asian countries. This study attempts to investigate the bioactive compounds in FES. First, FES extract was separated into fractions to facilitate the investigation and 72 compounds were identified using LC–MSn. Subsequently, Immune-enhancement effects of FES and its components were investigated on bone marrow cells and neuroprotective effects against H2O2 induced oxidative damage were evaluated in SH-SY5Y neuroblastoma cells and bEnd.3. Our results indicated that fraction 3, 5, 6 and 8 showed significant improvements on immune function, while several fractions had cytoprotective effects against H2O2-induced oxidative injury. Jionoside A1 isolated from Radix Rehmanniae Praeparata displayed dose dependent immune-enhancement activity. 20(R)-ginsenoside Rg3 could protect bEnd.3 against oxidative damage. Furthermore, echinacoside, jionoside A1, vitexin-2-O-rhamnoside, acteoside and isoacteoside possessed moderate protective activities on H2O2-treated SH-SY5Y cells. In conclusion, our study provided both chemical and biological evidences to support clinical application of FES.
Last. Jing Qian(ZJU: Zhejiang University)H-Index: 10
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Abstract Aims We aimed to investigate the effects of echinacoside (ECH) on hematopoietic function in 5-FU-induced bone marrow depression mice. Main methods In vitro , after stimulation with ECH, the proliferation ability of bone marrow (BM) cells and bone marrow stromal cells (BMSCs) derived from myelosuppression mice were assessed by CCK8 assay and morphology, respectively. In vivo , 5-FU-induced myelosuppression or control mice were intragastrically administrated with either ECH at 15 mg/kg or...
Last. Yiyu Cheng(ZJU: Zhejiang University)H-Index: 36
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In comparison with monotherapy in western medicine, traditional Chinese medicine (TCM) advocates combinational therapy for treating diseases and TCM formula is a representative for this approach. Despite of extensive clinical applications of TCM formulae, knowledge about their pharmacological activities, mechanisms of action and cellular targets remains limited. A main contributing factor to these unanswered questions is unavailability of chemical compositions and their contents in the formulae....
Abstract Food supplements based on Vitex agnus castus L. (Verbenaceae) fruits, also known as chasteberry, are routinely used by women against somatic and psychic premenstrual symptoms such as depression, sadness or irritability. With the aim of highlighting the differences in the chemical profiles of cultivated fruits and different parts of wild plants (fruits, leaves and sprouts) of V. agnus castus , a method concerning with the quali–quantitative study of the derived hydroalcoholic extracts wa...
#1Ping Shao(Zhejiang University of Technology)H-Index: 22
#2J. F. Zhang(Zhejiang University of Technology)H-Index: 2
Last. Sun Peilong(Zhejiang University of Technology)H-Index: 3
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Abstract Chlorogenic acid (CGA) is a polyphenol commonly existed in fruits and vegetables with a variety of bioactivities. To increase its stability and potential broader industrial application, CGA/cyclodextrin (CD) complexes were prepared using β-cyclodextrin (β-CD) and its derivative (2-hydroxypropyl)-β-cyclodextrin (2-HP-β-CD). Their inclusion complexation behavior and characterization were investigated by FT-IR, XRD, SEM, and 1 H NMR spectroscopy. The morphologic and spectral studies indica...
Last. Andreas Hensel(WWU: University of Münster)H-Index: 37
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Abstract Ethnopharmacological relevance The aerial parts of Lippia integrifolia (Gris.) Hieronymus (Verbenaceae), known as incayuyo , are used by the peasant population of Argentina for treatment of diseases related to a gastrointestinal system, mainly for inflammation of the stomach and have also been included into the Argentina Food Code. This study aimed to investigate the phytochemical profile of hydrophilic extracts from the herbal material by LC−MS and to evaluate potential pharmacological...
#1Jong Seok Lee(GSU: Georgia State University)H-Index: 20
#2Hye Suk Hwang(GSU: Georgia State University)H-Index: 17
Last. Sang-Moo Kang(GSU: Georgia State University)H-Index: 5
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Ginseng herbal medicine has been known to have beneficial effects on improving human health. We investigated whether red ginseng extract (RGE) has preventive effects on influenza A virus infection in vivo and in vitro. RGE was found to improve survival of human lung epithelial cells upon influenza virus infection. Also, RGE treatment reduced the expression of pro-inflammatory genes (IL-6, IL-8) probably in part through interference with the formation of reactive oxygen species by influenza A vir...
#1Wen-xia Zheng(WUST: Wuhan University of Science and Technology)H-Index: 4
#2Feng Wang(WUST: Wuhan University of Science and Technology)H-Index: 16
Last. Yu-ying Sun(WUST: Wuhan University of Science and Technology)H-Index: 1
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AbstractPrimary objective: To examine the neuroprotection of baicalin, a flavonoid compound derived from the dried root of Scutellaria baicalensis Georgi, on neurons.Research design: A rat PC12 cell line was used to study the neuroprotection and possible mechanisms of baicalin on H2O2-induced neuron damage.Methods: Three anti- and one pro-apoptosis genes in PC12 cells were examined. Cell apoptosis was induced by H2O2 and apoptotic rate was obtained by flow cytometry. MTT for cell viability, immu...
#1Guo-Bo Xu(CAS: Chinese Academy of Sciences)H-Index: 2
#2Ni-Ni Wang(CAS: Chinese Academy of Sciences)H-Index: 3
Last. Guo-You Li(CAS: Chinese Academy of Sciences)H-Index: 17
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Seven new orsellinic acid esters, 1-7, and four known compounds were isolated from the solid, fermented rice culture of Chaetomium globosporum (cib-132). Their structures were elucidated by 1D- and 2D-NMR spectra, and the relative configuration of compound 1 was determined by X-ray crystallographic analysis.
Last. De-Jun Hu(UM: University of Macau)H-Index: 15
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Abstract Root of Panax notoginseng (Burk.) F.H. Chen ( Sanqi in Chinese) is one of traditional Chinese medicines (TCMs) based functional food. Saponins are the major bioactive components. The shortage of reference compounds or chemical standards is one of the main bottlenecks for quality control of TCMs. A novel strategy, i.e. standardized reference extract based qualification and single calibrated components directly quantitative estimation of multiple analytes, was proposed to easily and effec...
Last. C.M. Wang(HK: Hungkuang University)H-Index: 1
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Abstract Various neurodegenerative diseases e.g. , Alzheimer's affect many elderly people that eventually leads to disability and premature death. No effective treatment for these neurodegenerative diseases has been developed yet. Oxidative stress-mediated neurodegeneration is one of the key pathophysiological factors involved in these diseases. Since numerous botanic components are well-known potent antioxidants, in this study, we aim to examine the neuroprotective effects of phytochemical comp...
#1Fei Luan(CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 5
#2Yafei Ji(CDUTCM: Chengdu University of Traditional Chinese Medicine)
Last. Nan Zeng(CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 2
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Abstract Codonopsis pilosula (Franch.) Nannf., as a well-known homology plant of medicine and food, has the function of replenishing the Qi, strengthening the spleen and tonifying the lung, nourishing the blood and engendering the liquid in traditional Chinese medicine. Accumulating evidence has demonstrated that the C. pilosula polysaccharides (CPPs) are one of major and representative pharmacologically active macromolecules and present multiple biological activities both in vitro and in vivo m...
Last. Ren Sun(UCLA: University of California, Los Angeles)H-Index: 64
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Martynoside (MAR) is a bioactive glycoside of Rehmannia glutinosa, a traditional Chinese herb frequently prescribed for treating chemotherapy-induced pancytopenia. Despite its clinical usage in China for thousands of years, the mechanism of MAR's hematopoietic activity and its impact on chemotherapy-induced antitumor activity are still unclear. Here, we showed that MAR protected ex vivo bone marrow cells from 5-fluorouracil (5-FU)-induced cell death and inflammation response by down-regulating t...
Donkeys (Equus asinus) face a global crisis. The health, welfare, and even survival of donkeys are being compromised as the demand for their skins increases. It is driven by the production of ejiao, a traditional Chinese remedy believed by some to have medicinal properties. It is estimated that the ejiao industry currently requires approximately 4.8 million donkey skins per year. Since there is no productive chain for donkey skin production outside of China, the activity is extractive and has re...
Last. Zhong Wang(Peking Union Medical College)H-Index: 18
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Abstract Ethnopharmacological relevance Precise target distribution is a key issue for further appreciate applications of fangjis (formulas) with similar efficacy and herbal constituents to maximize efficacy and minimize toxicity. Aim of the study To develop an algorithm for investigating the differential target distributions and characteristic mechanisms of fangjis. Main methods In this study, we proposed a Modulome-Fangjiome Association Study (MoFAS) for comparing fangjis from qi-invigorating ...
Phenylethanoid glycosides (PhGs) are compounds made of phenylethyl alcohol, caffeic acid and glycosyl moieties. The first published references about phenylethanoid glycosides concerned the isolation of echinacoside from Echinaceu ungustifolia (Asteraceae) in 1950 and verbascoside from Verbascum sinuatum (Scrophulariaceae) in 1963. Over the past 60 years, many compounds with these structural characteristics have been isolated from natural sources, and most of these compounds possess significant b...
#1Xue-mei Tang(CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
#2Jin-lin Guo(CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
Last. Paul C. Ho(NUS: National University of Singapore)H-Index: 56
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Abstract Different therapeutically active ingredients, from plants, animals, and mineral sources, are prescribed as traditional Chinese medicines (TCM). TCMs, from animal sources, are rich in proteins and peptides. Different advanced proteomics technologies, such as two-dimensional gel electrophoresis (2-DE), multi-dimensional liquid chromatography (MDLC), matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), and isobaric tags for relative and absolute quan...
Panax notoginseng (Burk.) F. H. Chen is a medicinal herb used to treat blood disorders since ancient times, of which the steamed form exhibits the anti-anemia effect and acts with a “blood-tonifying” function according to traditional use. The present study aimed to investigate the anti-anemia effect and underlying mechanism of steamed Panax notogensing (SPN) on mice with blood deficiency syndrome (BDS) induced by chemotherapy. BDS was induced in mice by cyclophosphamide and acetylphenylhydrazine...
Abstract Ginseng has been used as a popular herbal medicine in East Asia for at least two millennia. However, 20(R)-ginseng saponins, one class of important rare ginsenosides, are rare in natural products. 20(R)-ginseng saponins are generally prepared by chemical epimerization and microbial transformation from 20(S)-isomers. The C20 configuration of 20(R)-ginseng saponins are usually determined by 13C NMR and X-ray single-crystal diffraction. 20(R)-ginseng saponins have antitumor, antioxidative,...
Qixianqingming granules (QXQM) comprise a traditional Chinese medicine (TCM) formula that was developed based on the combination of TCM theory and clinical practice. This formula has been proven to effectively treat asthma. In this study, an analytical procedure using ultraperformance liquid chromatography, coupled with electrospray ionization quadrupole time-of-flight mass spectrometry, was established for the rapid separation and sensitive identification of the chemical components in QXQM and ...
Last. Haibin Qu(ZJU: Zhejiang University)H-Index: 30
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Abstract Ethnopharmacological relevance Fufang E'jiao Jiang (FEJ), a famous traditional Chinese medicine formula from “Liangyi Ointment”, consists of five crude drugs, Colla corii asini, Radix Ginseng Rubra, Radix Rehmanniae Preparata, Codonopsis pilosula, and Crataegus pinnatifida Bge. It has pronounced functions of qi-nourishing and blood-activating. Recently, it has been widely used in China as a medication against myelosuppression in cancer treatment. Aim of the study We aimed to investigate...
