Comparative study of the osteogenic ability of four different ceramic constructs in an ectopic large animal model.

Published on Mar 1, 2016in Journal of Tissue Engineering and Regenerative Medicine3.078
· DOI :10.1002/TERM.1782
Véronique Viateau14
Estimated H-index: 14
(École nationale vétérinaire d'Alfort),
Mathieu Manassero3
Estimated H-index: 3
(École nationale vétérinaire d'Alfort)
+ 5 AuthorsMorad Bensidhoum21
Estimated H-index: 21
(University of Paris)
Sources
Abstract
Tissue-engineered constructs combining bone marrow mesenchymal stem cells with biodegradable osteoconductive scaffolds are very promising for repairing large segmental bone defects. Synchronizing and controlling the balance between scaffold-material resorption and new bone tissue formation are crucial aspects for the success of bone tissue engineering. The purpose of the present study was to determine, and compare, the osteogenic potential of ceramic scaffolds with different resorbability. Four clinically relevant granular biomaterial scaffolds (specifically, Porites coral, Acropora coral, beta-tricalcium phosphate and banked bone) with or without autologous bone marrow stromal cells were implanted in the ectopic, subcutaneous-pouch sheep model. Scaffold material resorption and new bone formation were assessed eight weeks after implantation. New bone formation was only detected when the biomaterial constructs tested contained MSCs. New bone formation was higher in the Porites coral and Acropora coral than in either the beta-tricalcium phosphate or the banked bone constructs; furthermore, there was a direct correlation between scaffold resorption and bone formation. The results of the present study provide evidence that, among the biomaterials tested, coral scaffolds containing MSCs promoted the best new bone formation in the present study. Copyright © 2013 John Wiley & Sons, Ltd.
📖 Papers frequently viewed together
68 Citations
30 Citations
2015
4 Authors (Tea Andric, ..., Joseph W. Freeman)
8 Citations
References41
Newest
#1Yoke Chin Chai (Katholieke Universiteit Leuven)H-Index: 21
#2Aurélie Carlier (Katholieke Universiteit Leuven)H-Index: 13
Last. Frank P. Luyten (Katholieke Universiteit Leuven)H-Index: 88
view all 8 authors...
Abstract Calcium phosphate (CaP) has traditionally been used for the repair of bone defects because of its strong resemblance to the inorganic phase of bone matrix. Nowadays, a variety of natural or synthetic CaP-based biomaterials are produced and have been extensively used for dental and orthopaedic applications. This is justified by their biocompatibility, osteoconductivity and osteoinductivity (i.e. the intrinsic material property that initiates de novo bone formation), which are attributed ...
152 CitationsSource
#1U. van der PolH-Index: 1
#2L. Mathieu (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 8
Last. Dominique P. Pioletti (EPFL: École Polytechnique Fédérale de Lausanne)H-Index: 37
view all 8 authors...
Previous studies support resorbable biocomposites made of poly L-lactic acid (PLA) and β-tricalcium phosphate (TCP) produced by supercritical gas foaming as a suitable scaffold for tissue engineering. The present study was undertaken to demonstrate the biocompatibility and osteoconductive properties of such a scaffold in a large animal cancellous bone model. The biocomposite (PLA/TCP) was compared with a currently used β-TCP bone substitute (ChronOSTM, Dr.Robert Mathys Foundation) representing a...
67 CitationsSource
#1Huipin Yuan (UT: University of Twente)H-Index: 37
#2Hugo Fernandes (UT: University of Twente)H-Index: 17
Last. Joost D. de Bruijn (QMUL: Queen Mary University of London)H-Index: 41
view all 9 authors...
Biomaterials can be endowed with biologically instructive properties by changing basic parameters such as elasticity and surface texture. However, translation from in vitro proof of concept to clinical application is largely missing. Porous calcium phosphate ceramics are used to treat small bone defects but in general do not induce stem cell differentiation, which is essential for regenerating large bone defects. Here, we prepared calcium phosphate ceramics with varying physicochemical and struc...
555 CitationsSource
#1Manitha B. NairH-Index: 17
#2Harikrishna VarmaH-Index: 31
Last. Annie JohnH-Index: 18
view all 4 authors...
Segmental bone defects caused by tumor resections, trauma, and skeletal abnormalities such as osteomyelitis remain a major problem in orthopedics because of the lack of predictability in attaining functional bone after the treatment. The objective of this study was to propose an indigenous porous biodegradable triphasic ceramic (calcium silicate, tricalcium phosphate, and hydroxyapatite [HA])-coated HA (core) (HASi) for the repair of such segmental defects. With respect to the synthesis of HASi,...
21 CitationsSource
#1Véronique Viateau ('ENS Paris': École Normale Supérieure)H-Index: 14
#2Morad Bensidhoum (Paris Diderot University)H-Index: 21
Last. Philippe PélissierH-Index: 1
view all 7 authors...
Abstract Animal experiments using the induced membrane procedure for bone tissue engineering purposes have provided evidence that the membrane has structural characteristics and biologic properties that may be used for bone tissue engineering purposes. Clinically relevant animal models have demonstrated that standardized particulate bone constructs can be used to repair large bone defects using the procedure and that the osteogenic ability of these constructs partially approaches that of bone au...
77 CitationsSource
#1Kaj Klaue (University of Bern)H-Index: 15
#2Ulf Knothe (Cleveland Clinic)H-Index: 18
Last. Stephan M. PerrenH-Index: 35
view all 7 authors...
Abstract Regeneration of living tissue varies with species, age and type of tissue, and undoubtedly with the biological and mechanical environment of the precise tissue. Autologous cancellous bone grafting is a well-known technique that provides bony regeneration. We investigated the efficiency of autologous bone grafting in a well-vascularised muscle environment, and additionally when isolated from the muscle and connected only to the bony environment. We designed a reproducible animal model pr...
79 CitationsSource
#1Sylvain Catros (French Institute of Health and Medical Research)H-Index: 20
#2N. Zwetyenga (French Institute of Health and Medical Research)H-Index: 1
Last. Jean-Christophe Fricain (French Institute of Health and Medical Research)H-Index: 23
view all 7 authors...
Induced Membranes Technique was first described to enhance bone reconstruction of large osseous defects. Previous in vitro studies established their osteoinductive potential, due to the presence of opteoblasts precursors and to high amounts of growth factors contained within. The purpose of this study was to test in vivo the osteoinductive properties of induced membranes on a macroporous HA-TCP in a nonosseous subcutaneous site. Subcutaneous-induced membranes were obtained in 21 rabbits; 1 month...
25 CitationsSource
Tissue engineering techniques have been proven effective in bone regeneration and repairing load-bearing bone defects. Previous studies, however, have heretofore been limited to the use of slowdegradable or natural biomaterials as scaffolds. There are, however, no reports on using biodegradable, synthetic beta-tricalcium phosphate (β-TCP) as scaffolds to repair weight-bearing bone defects in large animals. In the present study, highly porous β-TCP scaffolds prepared by the polymeric sponge metho...
137 CitationsSource
#1Masanobu Kamitakahara (Tohoku University)H-Index: 25
#2Chikara OhtsukiH-Index: 59
Last. Toshiki MiyazakiH-Index: 29
view all 3 authors...
Various calcium phosphates are used for bone repair. Although hydroxyapatite (HA) sintered ceramics are widely used due to their osteoconductivity, its bioresorbability is so low that HA remains in the body for a long time after implantation. In contrast, tricalcium phosphate (TCP) ceramics show resorbable characters during bone regeneration, and can be completely substituted for the bone tissue after stimulation of bone formation. Therefore, much attention is paid to TCP ceramics for scaffold m...
262 CitationsSource
#1Ranieri CanceddaH-Index: 82
#2Paolo GiannoniH-Index: 20
Last. Maddalena MastrogiacomoH-Index: 14
view all 3 authors...
Abstract The repair of large segmental bone defects due to trauma, inflammation and tumor surgery remains a major clinical problem. Animal models were developed to test bone repair by tissue engineering approaches, mimicking real clinical situations. Studies differed with regard to animals (dog, sheep, goat), treated bone (femur, tibia, mandible), chemistry and structure of the scaffolds. Still, an advantage in the bone formation and in the healing of the segmental defect was always observed whe...
478 CitationsSource
Cited By18
Newest
#1Xiaoyu Cai (Saarland University)H-Index: 2
#1Xiaoyu Cai (Saarland University)H-Index: 10
Last. Henning Madry (Saarland University)H-Index: 57
view all 4 authors...
Abstract Background Articular cartilage is an extremely specialized connective tissue which covers all diarthrodial joints. Implantation of chondrogenic cells without or with additional biomaterial scaffolds in ectopic locationsin vivo generates substitutes of cartilage with structural and functional characteristics that are used in fundamental investigations while also serving as a basis for translational studies. Methods Literature search in Pubmed. Results and discussion This narrative review...
Source
#1Karl Norris (Lancaster University)H-Index: 2
#2Magdalena Kocot (AGH University of Science and Technology)H-Index: 2
Last. Timothy Douglas (Lancaster University)H-Index: 25
view all 11 authors...
Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and ability to enhance proliferation and osteogenic differentiation of bone-forming cells. Hence, they have promise in the area of bone tissue regeneration. In contrast to commonly used ceramic minerals for bone regener...
2 CitationsSource
#1Dhanak Gupta (University of Birmingham)H-Index: 2
#1Dhanak Gupta (University of Birmingham)H-Index: 4
Last. Timothy Douglas (Lancaster University)H-Index: 25
view all 9 authors...
Abstract This work explores novel biocomposite hydrogels fabricated using 40% (wt/vol) solution of whey protein isolate (WPI, from the food industry) mixed with increasing concentrations of synthetic aragonite rod-like powder of 0, 100, 200 and 300 mg/ml (named WPI0, WPI100, WPI200 and WPI300). FTIR results showed that aragonite was successfully incorporated into the WPI hydrogel network. SEM and micro-CT investigations revealed that aragonite was mainly concentrated near the edges of the compos...
5 CitationsSource
#1Florian Andreas Probst (LMU: Ludwig Maximilian University of Munich)H-Index: 16
#2Riham Fliefel (LMU: Ludwig Maximilian University of Munich)H-Index: 8
Last. Sven Otto (LMU: Ludwig Maximilian University of Munich)H-Index: 27
view all 11 authors...
Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used for bone regeneration facilitating the formation of bone extracellular matrix to promote reparative osteogenesis. The present study assessed the potential of cell-scaffold constructs for the regeneration of extensive mandibular bone defects in a minipig model. Sixte...
12 CitationsSource
#1Ana S. Neto (University of Aveiro)H-Index: 4
#2José M.F. Ferreira (University of Aveiro)H-Index: 77
Bone is a vascularized and connective tissue. The cortical bone is the main part responsible for the support and protection of the remaining systems and organs of the body. The trabecular spongy bone serves as the storage of ions and bone marrow. As a dynamic tissue, bone is in a constant remodelling process to adapt to the mechanical demands and to repair small lesions that may occur. Nevertheless, due to the increased incidence of bone disorders, the need for bone grafts has been growing over ...
25 CitationsSource
#1Walter Bonani (University of Trento)H-Index: 14
#2Weerasak Singhatanadgige (Chula: Chulalongkorn University)H-Index: 8
Last. Antonella Motta (University of Trento)H-Index: 42
view all 4 authors...
Materials selection is a critical aspect for the production of scaffolds for osteochondral tissue engineering. Synthetic materials are the result of man-made operations and have been investigated for a variety of tissue engineering applications. Instead, the products of physiological processes and the metabolic activity of living organisms are identified as natural materials. Over the recent decades, a number of natural materials, namely, biopolymers and bioceramics, have been proposed as the ma...
7 CitationsSource
#1Sevil Köse (Atılım University)H-Index: 8
#2Berna KankilicH-Index: 5
Last. Feza Korkusuz (Hacettepe University)H-Index: 11
view all 7 authors...
Bioceramics are type of biomaterials generally used for orthopaedic applications due to their similar structure with bone. Especially regarding to their osteoinductivity and osteoconductivity, they are used as biodegradable scaffolds for bone regeneration along with mesenchymal stem cells. Since chemical properties of bioceramics are important for regeneration of tissue, physical properties are also important for cell proliferation. In this respect, several different manufacturing methods are us...
5 CitationsSource
#1Miryam Mebarki (French Institute of Health and Medical Research)H-Index: 5
#2Laura CoquelinH-Index: 4
Last. Nathalie ChevallierH-Index: 35
view all 8 authors...
In order to induce an efficient bone formation with human bone marrow mesenchymal stromal cells (hBMSC) associated to a scaffold, it is crucial to determine the key points of the hBMSC action after in vivo transplantation as well as the appropriate features of a scaffold. To this aim we compared the hBMSC behavior when grafted onto two biomaterials allowing different bone potential in vivo. The cancellous devitalized Tutoplast®-processed bone (TPB) and the synthetic hydroxyapatite/β-tricalcium-p...
37 CitationsSource
#1A. Decambron (École nationale vétérinaire d'Alfort)H-Index: 3
#2M. Manassero (École nationale vétérinaire d'Alfort)H-Index: 7
Last. V. Viateau (École nationale vétérinaire d'Alfort)H-Index: 9
view all 7 authors...
Objectives To compare the therapeutic potential of tissue-engineered constructs (TECs) combining mesenchymal stem cells (MSCs) and coral granules from either Acropora or Porites to repair large bone defects. Materials and Methods Bone marrow-derived, autologous MSCs were seeded on Acropora or Porites coral granules in a perfusion bioreactor. Acropora -TECs (n = 7), Porites -TECs (n = 6) and bone autografts (n = 2) were then implanted into 25 mm long metatarsal diaphyseal defects in sheep. Bimont...
6 CitationsSource
#1Timothy Douglas (UGent: Ghent University)H-Index: 25
#2Katarzyna Sobczyk (AGH University of Science and Technology)H-Index: 1
Last. Andre G. Skirtach (UGent: Ghent University)H-Index: 58
view all 21 authors...
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel–microparticle composites, containing Zn, Ca and Mg. Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg. Microp...
9 CitationsSource