Comparative Evaluation of Two Methods for Preparative Fractionation of Proteinaceous Subvisible Particles—Differential Centrifugation and FACS

The goal of this study was to compare and evaluate two preparative techniques for fractionation of proteinaceous subvisible particles. This work enables future studies to address the potential biological consequences of proteinaceous subvisible particles in protein therapeutic products. Particles were generated by heat stress and separated by size using differential centrifugation and FACS (Fluorescence-activated cell sorter). Resulting...