Hyperbaric oxygen enhances neutrophil apoptosis and their clearance by monocyte-derived macrophages

Published on Jun 9, 2015in Biochemistry and Cell Biology2.46
· DOI :10.1139/BCB-2014-0157
Anwar J. Almzaiel2
Estimated H-index: 2
(University of Plymouth),
Anwar J. Almzaiel2
Estimated H-index: 2
(University of Plymouth)
+ 1 AuthorsA. John Moody23
Estimated H-index: 23
(PSU: Plymouth State University)
Sources
Abstract
Neutrophil apoptosis and clearance by macrophages are essential for wound healing. Evidence suggests that hyperbaric oxygen (HBO) exposure may enhance neutrophil apoptosis, but HBO effects leading to neutrophil clearance by macrophages are still unclear. In the current study, bovine neutrophils and monocyte-derived macrophages (MDMΦ) were co-cultured under HBO (97.9% O2, 2.1% CO2 at 2.4 atm absolute (ATA)) (1 atm = 101.325 kPa), hyperbaric normoxia (8.8% O2 at 2.4 ATA), normobaric hyperoxia (95% O2, 5% CO2), normoxia (air), and normobaric hypoxia (5% O2, 5% CO2). Phagocytosis of fresh and 22 h aged neutrophils by MDMΦ was increased after HBO pre-treatment, assessed using flow cytometry and light microscopy. Enhanced clearance of neutrophils was accompanied by an increase in H2O2 levels following HBO pre-treatment with upregulation of IL-10 (anti-inflammatory cytokine) mRNA expression in LPS-stimulated MDMΦ that had ingested aged neutrophils. TNF-α (pro-inflammatory cytokine) gene expression did not change...
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