Gene

Catalysis

Photosensitizer

Biotechnology

Bacteria

Biology

Photodynamic therapy

Photochemistry

Materials science

Reactive oxygen species

Antibacterial activity

Biochemistry

Wavelength

Genetics

In vivo

Physics

Organic chemistry

Optics

Metallacycle

Excitation wavelength

Microbiology

Fluorescence

Escherichia coli

Ruthenium

Chemistry

X-ray crystallography

Biophysics

Diffraction

Optoelectronics

Proceedings of the National Academy of Sciences

Ruthenium (Ru) complexes are developed as latent emissive photosensitizers for cancer and pathogen photodiagnosis and therapy. Nevertheless, most existing Ru complexes are limited as photosensitizers in terms of short excitation and emission wavelengths. Herein, we present an emissive Ru(II) metallacycle (herein referred to as 1) that is excited by 808-nm laser and emits at a wavelength of ∼1,000 nm via coordination-driven self-assembly. Metallacycle 1 exhibits good optical penetration (∼7 mm) and satisfactory reactive oxygen species production properties. Furthermore, 1 shows broad-spectrum antibacterial activity (including against drug-resistant Escherichia coli) as well as low cytotoxicity to normal mammalian cells. In vivo studies reveal that 1 is employed in precise, second near-infrared biomedical window fluorescent imaging-guided, photo-triggered treatments in Staphylococcus aureus-infected mice models, with negligible side effects. This work thus broads the applications of supramolecular photosensitizers through the strategy of lengthening their wavelengths.

Long wavelength–emissive Ru(II) metallacycle–based photosensitizer assisting in vivo bacterial diagnosis and antibacterial treatment