Tetracycline

Antibiotics

Bacteria

Biology

Microbiology

Multiple drug resistance

Listeria

Sulfamethoxazole

Erythromycin

Listeria monocytogenes

Genetics

Antibiotic resistance

PLoS ONE

The occurrence and the antibiogram signatures of Listeria monocytogenes (Lm) recovered from 65 milk samples and its products within the Eastern Cape province were examined. The EN ISO 11290:2017 procedures Parts 1 and 2 described by the International Organization for Standardization for the enumeration and isolation of Lm was adopted for the study. Lm was detected in 18.46% of all the samples examined, and the strains recovered from the samples belong to serotypes 4b and 1/2b. The virulence determinants including prf A, plc A, plc B, inl A, inl C, hly , mpl , act A, inl J and inl B were detected in all the isolates. About 95.24% of the studied Lm isolates demonstrated potential capacity for biofilm formation. The antibiogram profile revealed high resistance against sulfamethoxazole (71.43%), trimethoprim (52.86%); erythromycin, cefotetan and oxytetracycline (42.86% respectively). About 85.71% exhibited multiple antibiotic resistance phenotypes against the test antibiotics. The resistance determinants encoding resistance against the β-lactamase antibiotics [such as the bla TEM , bla SHV , bla TEM variants (TEM-1 and TEM-2) and the bla Z ], the tetracycline resistance genes (including tet A, tet D, tet G and tet M and tetK ) were detected among resistant isolates. In addition, the aminoglycoside resistance gene aph (3)-IIa (aphA2) a was detected only in one isolate. Finally, the sulfonamide resistance genes including the sul 2 and the sul 1 genes were the most frequently observed among Lm isolates. Generally, 71.43% of all Lm isolates recovered from the samples investigated harboured one or more resistance genes encoding resistance against various antibiotics. The antibiogram signatures of Lm isolates observed in this study is an indication that empirical treatment of listeriosis may be challenging in the future as the pathogen may obliterate the success of antibiotics. We, therefore, advocate for the recognition of the One Health approach to ensuring food safety and curbing the spread of antimicrobial resistance in food.

Assessment of multidrug-resistant Listeria monocytogenes in milk and milk product and One Health perspective