The major processes in the extraction of C. oleifera oil. In recent years, the need for high-quality and high-quantity production of C. oleifera oil for human consumption has been increased. The present review examines the chemical composition of C. oleifera oil, bioactive substances, extraction technologies, and evidences supporting the health benefits of C. oleifera oil. Emerging technologies such as aqueous extraction, and supercritical fluid extraction are highly efficient processes, and can achieve higher recovery while reducing solvent and energy consumption. This review provides an in-depth discussion on the various extraction technologies and factors affecting the extraction efficiency of C. oleifera oil using traditional and emerging methods. The In this review, chemical composition of C. oleifera oil and evidences supporting the health benefits of C. oleifera oil are briefly introduced. Conventional and innovative extraction technologies for C. oleifera oil production are systematically reviewed. Furthermore, the influences of different extraction methods on the C. oleifera oil characteristics are also introduced. Furthermore, challenges and future prospects of extraction of C. oleifera oil have been identified and discussed. • The chemical composition of C. oleifera oils was reviewed. • The traditional and emerging extraction technologies of C. oleifera oils were reviewed. • The influences of extraction methods on the quality of C. oleifera oil were first systematically reviewed. • The prospects of C. oleifera oil extraction has been identified and discussed. C. oleifera oil is one of the high-quality edible oils recommended by the Food and Agriculture Organization of the United Nations (FAO). Pharmacological studies have shown that C. oleifera oil is the homology of medicine and food, and it possesses extensive beneficial health properties both in vivo and in vitro. C. oleifera oil found its application in the functional food, cosmetic, and pharmaceutical industries. In recent years, the need for high...