Health benefits

Biotechnology

Bacteria

Biology

Chromatography

Probiotic

Biochemistry

Spray drying

Food science

Gastrointestinal transit

Genetics

Fermented milk products

Lactic acid

Microbiology

Medicine

Chemistry

Cell

Gastroenterology

Viability assay

Traditional medicine

Beni-Suef University Journal of Basic and Applied Sciences

Background Probiotics are live microbial supplements known for its health benefits. Consumption of probiotics reported to improve several health benefits including intestinal flora composition, resistance against pathogens. In the recent years, there is an increasing trend of probiotic-based food products in the market. Main body Probiotics cells are targeted to reach the large intestine, and the probiotics must survive through the acidic conditions of the gastric environment. It is recommended to formulate the probiotic bacteria in the range of 10 8 –10 9 cfu/g for consumption and maintain the therapeutic efficacy of 10 6 –10 7 cfu/g in the large intestine. During the gastrointestinal transit, the probiotics will drastically lose its viability in the gastric environment (pH 2). Maintaining cell viability until it reaches the large intestine remains challenging task. Encapsulating the probiotics cells with suitable wall material helps to sustain the survival of probiotics during industrial processing and in gastrointestinal transit. In the encapsulation process, cells are completely enclosed in the wall material, through different techniques including spray drying, freeze drying, extrusion, spray freeze drying, emulsification, etc. However, spray-drying and freeze-drying techniques are successfully used for the commercial formulation; thus, we limited to review those encapsulation techniques. Short conclusions The survival rate of spray-dried probiotics during simulated digestion mainly depends on the inlet air temperature, wall material and exposure in the GI condition. And fermentation, pH and freeze-drying time are the important process parameters for maintaining the viability of bacterial cells in the gastric condition. Improving the viability of probiotic cells during industrial processing and extending the cell viability during storage and digestion will be the main concern for successful commercialization. Graphical abstract

Encapsulation of probiotics: past, present and future