DNA replication factor CDT1

Origin of replication

Biology

Gene

Control of chromosome duplication

Semiconservative replication

Helicase

Minichromosome maintenance

Origin recognition complex

Replication factor C

Pre-replication complex

DNA replication

Cell biology

Licensing factor

Genetics

DNA re-replication

Eukaryotic DNA replication

Annual Review of Biochemistry

DNA replication in eukaryotic cells initiates from large numbers of sites called replication origins. Initiation of replication from these origins must be tightly controlled to ensure the entire genome is precisely duplicated in each cell cycle. This is accomplished through the regulation of the first two steps in replication: loading and activation of the replicative DNA helicase. Here we describe what is known about the mechanism and regulation of these two reactions from a genetic, biochemical, and structural perspective, focusing on recent progress using proteins from budding yeast.

The Initiation of Eukaryotic DNA Replication