Wastewater

Engineering

Sewage treatment

Ecology

Bacteria

Biology

Salinity

Nitrogen

Ammonium

Nitrification

Genetics

Environmental engineering

Organic chemistry

Halophile

Environmental chemistry

Environmental science

Nitrite

Chemistry

Pulp and paper industry

Nitrate

The Science of The Total Environment

The ammonium-assimilating biosystem is a promising solution to improve the susceptible biological nitrogen removal (BNR) and to achieve nitrogen recovery in saline wastewater treatment. However, the treatment performance and functional stability of ammonium-assimilating biosystems have not been fully illuminated in hypersaline wastewater. In this study, although the dramatic decrease of removal efficiency of NH4+-N and PO43--P was observed in ammonium-assimilating biosystems under the salinity from 3% to 7%, the direction of nitrogen conversions through assimilation was insusceptible to high salinity. The extremely low concentrations of nitrite and nitrate accumulation and abundances of nitrification functional genes confirmed that the process of nitrification was negligible in all biosystems. Ammonium-assimilating biosystems maintained robustness and functional stability in hypersaline wastewater. The increase of salinity stimulated the production of EPS and changed the microbial community by enriching Proteobacteria and halophilic genera. We anticipate that the ammonium-assimilating biosystem could be a promising strategy for hypersaline wastewater treatment and future practical applications.

The effect of salinity on ammonium-assimilating biosystems in hypersaline wastewater treatment