Gene

Helicobacter pylori

Gastric biopsy

Biology

Gastritis

Helicobacter Infections

DNA sequencing

Antibiotic resistance

Genetics

Antibiotics

Microbiology

Internal medicine

Medicine

Gastroenterology

Helicobacter pylori cure rates have declined worldwide, making pretreatment susceptibility testing increasingly important.1–3 Many US diagnostic laboratories now offer culture and susceptibility testing. In addition, molecular-based testing using next-generation sequencing (NGS) is now available in the United States.4 NGS provides susceptibility profiling based on detection of mutations in the H pylori genome associated with phenotypic resistance.5 Advantages of NGS include detecting resistance to multiple antibiotics simultaneously and rapidly4 and providing results comparable with culture-based methods using either fresh or formalin-fixed gastric biopsies.

Comparable Results of Helicobacter pylori Antibiotic Resistance Testing of Stools vs Gastric Biopsies Using Next-Generation Sequencing