Original paper
Direct N- or C-Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach
Abstract
Site-specific protein labeling is important in biomedical research and biotechnology. While many methods allow site-specific protein modification, a straightforward approach for efficient N-terminal protein labeling is not available. We introduce a novel sortase-mediated swapping approach for a one-step site-specific N-terminal labeling with a near-quantitative yield. We show that this method allows rapid and efficient cleavage and simultaneous...
Paper Details
Title
Direct N- or C-Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach
Published Date
Nov 8, 2021
Journal
Volume
32
Issue
11
Pages
2397 - 2406
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Notes
History