Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites

Modified buffy coat method (BCM) using microhematocrit tubes and acridine orange (AO) staining permitsquick(~10 min/sample) and accurate diagnosis of commonbovine hemoparasites compared to the light microscopy(LM) of Giemsa stained smears.Babesia, Theileria,Anaplasma, Ehrlichia and Trypanosoma parasites were detected. It was easy to see Babesia merozoites, trypomastigotes and epimastigotes of Trypanosoma parasite. Similarly, fluorescent...