Protein–Ligand interactions for hydrophobic charge-induction chromatography: A QCM-D study

Hydrophobic charge-induction chromatography (HCIC) is a novel technology for antibody purification. The interaction between immunoglobulin G (IgG) and HCIC ligands was investigated by dissipative quartz crystal microbalance (QCM-D). HCIC ligands 2-mercapto-1-methylimidazole (MMI), 2-mercaptobenzimidazole (MBI) and 2-mercapto-4-methylpyrimidine hydrochloride (MMp) were coupled to the surface of a self-assembled monolayer (SAM) terminated by epoxy...