LRRK2 and WAVE2 regulate microglial‐transition through distinct morphological phenotypes to induce neurotoxicity in a novel two‐hit in vitro model of neurodegeneration

We report a novel in vitro classification system that tracks microglial activation state and their potential neurotoxicity. Mixed live-cell imaging was used to characterize transition through distinct morphological phenotypes, production of reactive oxygen species (ROS), formation of reactive microglial aggregates, and subsequent cytokine production. Transwell cultures were used to determine microglial migration (control and lipopolysaccharide...