Molecular identification and pathogenicity study of virulent Citrobacter freundii associated with mortality of farmed Labeo rohita (Hamilton 1822), in India

The present research study was carried out to investigate the causative agent responsible for infection and mortality of farmed Labeo rohita. Samples of infected fishes were collected from aquaculture farms of West Bengal, India. Biochemical and molecular identification revealed that isolated strain EM4 was Citrobacter freundii. Furthermore, a challenge study was carried out to determine the number of bacterial colonies required for 50%...