Charge-Tunable μ-Gelzymes Achieving Cellulose Affinity under Synergistic Ionic Liquid-Pretreated Biomass in Situ Saccharification

Enzyme-loaded microgels (μ-Gelzymes) have gained increasing attention for their facile fabrication, versatile applicability, and fine controllability. Herein, a series of poly(N-isopropylacrylamide) (PNIPAM)-based μ-Gelzymes (P-C) with a tunable surface potential were prepared to improve the cellulase biocatalytic effect. The P-C showed better cellulose affinity than free cellulase, highly reducing its nonproductive binding onto lignin, while...