Nanoparticle

Biology

Materials science

Fish <Actinopterygii>

Chromatography

Histamine

Fishery

Endocrinology

Biochemistry

Immunoassay

Immunology

Residue (chemistry)

Nanotechnology

Colloidal gold

Coefficient of variation

Mass spectrometry

Chemistry

Detection limit

Antibody

Hapten

Journal of Hazardous Materials

To ensure food quality and prevent histamine (HA) toxicity, a rapid and direct method of detecting HA is required. In this work, we prepared a monoclonal antibody (mAb) against HA using a hapten produced by the introduction of a phenyl-containing linker. The novel mAb exhibited high sensitivity against HA as determined by ELISA, with a half-maximal inhibitory concentration of 21.51 ng/mL. A gold nanoparticle-based immunosensor was fabricated for rapid detection of HA in fish samples. After optimizing the immunosensor, a visual limit of detection (LOD) and a calculated LOD were 0.25 mg/kg and 10.48 μg/kg for HA, respectively. Recovery rates from the spiked fish samples ranged from 87.33% to 104.67% with the coefficient of variation below 10.82%. Concurrently, the whole process in testing real sample was completed within 15 min, and all results were well confirmed and comparable by liquid chromatography-mass spectrometry and the commercial test strip. These data revealed that the proposed immunosensor could be used as a monitoring tool for the rapid and direct detection of HA in fish samples.

An immunochromatographic sensor for ultrasensitive and direct detection of histamine in fish