Combining direct urinary injection with automated filtration and nanoflow LC-MS for the confirmatory analysis of doping-relevant small peptide hormones

• Nanoflow LC-MS method for confirmation of doping-relevant small peptide hormones. • Direct urinary injection to minimize offline sample preparation. • Online automatic filtration and filter backflush hyphenated setup. • Robust nanoLC-MS assay validated for routine implementation. • Easily translated to other matrices and related analytical fields. Nano-liquid chromatography (nanoLC) has proven itself as a powerful tool and its scope entails...