Roles of Dental Mesenchymal Stem Cells in the Management of Immature Necrotic Permanent Teeth

Published on May 19, 2021in Frontiers in Cell and Developmental Biology5.201
· DOI :10.3389/FCELL.2021.666186
Dixin Cui5
Estimated H-index: 5
(Sichuan University),
Sihan Yu (Sichuan University)+ 5 AuthorsMian Wan10
Estimated H-index: 10
(Sichuan University)
Dental caries and trauma always lead to pulp necrosis and subsequent root development arrest of young permanent teeth. The traditional treatment, apexification, with absence of further root formation, results in abnormal root morphology and compromises long-term prognosis. Regeneration endodontics procedures (REPs) have been developed and considered as an alternative strategy for management of immature permanent teeth with pulpal necrosis, including cell-free REPs and cell-based REPs. Cell-free REPs, including revascularization and cell homing with molecules recruiting endogenous mesenchymal stem cells (MSCs), have been widely applied in clinical treatment, showing optimistic periapical lesion healing and continued root development. However, regenerated pulp-dentin complex is still absent in these cases. Dental MSCs, as one of the essentials of tissue engineering, are vital seed cells in regenerative medicine. Dental MSC-based REPs have presented promising potential with pulp-dentin regeneration in large animal studies and clinical trials via cell transplantation. In the present review, we summarize current understanding of the biological basis of clinical treatments for immature necrotic permanent teeth, the roles of dental MSCs during this process, and update the progress of MSC-based REPs in the administration of immature necrotic permanent teeth.
#1Ghada Beldi (French Institute of Health and Medical Research)H-Index: 2
#2Sheyda Bahiraii (University of Vienna)H-Index: 1
Last. Sina Naserian (French Institute of Health and Medical Research)H-Index: 10
view all 7 authors...
Mesenchymal stem cells (MSCs) have drawn lots of attention as gold standard stem cells in fundamental and clinical researches during the last 20 years. Due to their tissue and vascular repair capacities, MSCs have been used to treat a variety of degenerative disorders. Moreover, MSCs are able to modulate immune cells' functions, particularly T cells while inducing regulatory T cells (iTregs). MSCs are very sensitive to inflammatory signals. Their biological functions could remarkably vary after ...
6 CitationsSource
#1Ghada Beldi (French Institute of Health and Medical Research)H-Index: 2
#2Maryam Khosravi (University of Paris)H-Index: 1
Last. Sina Naserian (French Institute of Health and Medical Research)H-Index: 10
view all 7 authors...
BACKGROUND In addition to their multilineage potential, mesenchymal stem cells (MSCs) have a broad range of functions from tissue regeneration to immunomodulation. MSCs have the ability to modulate the immune response and change the progression of different inflammatory and autoimmune disorders. However, there are still many challenges to overcome before their widespread clinical administration including the mechanisms behind their immunoregulatory function. MSCs inhibit effector T cells and oth...
10 CitationsSource
#1Sina Naserian (French Institute of Health and Medical Research)H-Index: 10
#2Mohamed Essameldin Abdelgawad (Helwan University)H-Index: 7
Last. Georges Uzan (French Institute of Health and Medical Research)H-Index: 35
view all 8 authors...
BACKGROUND Endothelial progenitor cells (EPCs) are non-differentiated endothelial cells (ECs) present in blood circulation that are involved in neo-vascularization and correction of damaged endothelial sites. Since EPCs from patients with vascular disorders are impaired and inefficient, allogenic sources from adult or cord blood are considered as good alternatives. However, due to the reaction of immune system against allogenic cells which usually lead to their elimination, we focused on the exa...
12 CitationsSource
#1Pierfrancesco Pagella (UZH: University of Zurich)H-Index: 14
#2Shayee Miran (UZH: University of Zurich)H-Index: 3
Last. Thimios A. Mitsiadis (UZH: University of Zurich)H-Index: 54
view all 6 authors...
Mesenchymal stem cells (MSCs) have the capacity to self-renew and differentiate into specific cell types and are, therefore, key players during tissue repair and regeneration. The use of MSCs for the regeneration of tissues in vivo is increasingly being explored and already constitutes a promising alternative to existing clinical treatments. MSCs also exert paracrine and trophic functions, including the promotion of innervation that plays fundamental roles in regeneration and in restoration of t...
4 CitationsSource
#1Claudia Brizuela (University of Los Andes)H-Index: 7
#2G. Meza (University of Los Andes)H-Index: 2
Last. Maroun Khoury (University of Los Andes)H-Index: 29
view all 10 authors...
A randomized controlled phase I/II clinical trial was designed to evaluate the safety and efficacy of encapsulated human umbilical cord mesenchymal stem cells in a plasma-derived biomaterial for regenerative endodontic procedures (REPs) in mature permanent teeth with apical lesions. The trial included 36 patients with mature incisors, canines, or mandibular premolars showing pulp necrosis and apical periodontitis. Patients were randomly and equally allocated between experimental (REP) or convent...
7 CitationsSource
Aim: The aim of this study is to verify the type of scaffold effect on tissue engineering for dentine regeneration in animal models. Materials & methods: Strategic searches were conducted through MEDLINE/PubMed, Web of Science and Scopus databases. The studies were included with the following eligibility criteria: studies evaluating dentine regeneration, and being an in vivo study. Results: From 1392 identified potentially relevant studies, 15 fulfilled the eligibility criteria. All studies desc...
3 CitationsSource
#1Sang Yun JeongH-Index: 3
#2Soonchul LeeH-Index: 1
Last. Jongman YooH-Index: 8
view all 6 authors...
We developed a novel dentin-pulp-like organoid. It has both stem-cell and odontoblast characteristics using a mesenchymal cell lineage of human dental-pulp stem cells (hDPSCs). The mixture of hDPSCs and Matrigel was transferred into the maintenance medium (MM) and divided into four different groups according to how long they were maintained in the odontogenic differentiation medium (ODM). All organoids were harvested at 21 days and analyzed to find the optimal differentiation condition. To asses...
2 CitationsSource
#1Yoko Kawase-Koga (TMU: Tokyo Medical University)H-Index: 8
#2Yasuyuki Fujii (UConn: University of Connecticut)H-Index: 1
Last. Daichi Chikazu (TMU: Tokyo Medical University)H-Index: 19
view all 5 authors...
Abstract Introduction Cell-based therapies require an emerging alternative treatment using easily harvested cell sources. Neural stem cells derived from various tissues, including brain, bone marrow, skin and retina can give rise to both neurons and glial cells. Recently, human dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) were demonstrated to have mesenchymal stem cell-like abilities such as self-renewal and multi-lineage differentiation, including n...
2 CitationsSource
#1Mohannad AlasqahH-Index: 1
#2Sulthan Ibrahim Raja Khan (National Guard Health Affairs)
Last. Ahmed Jamleh (National Guard Health Affairs)H-Index: 8
view all 4 authors...
The regenerative endodontic procedure (REP) is considered a viable treatment option for immature teeth with necrotic pulp and periapical radiolucency which can facilitate continued root formation. In this report, an immature necrotic mandibular molar received REP in three appointments wherein chemomechanical debridement was performed with a sequential application of nonsetting calcium hydroxide (in the whole canal) and triple antibiotics paste (in the root’s middle third) dressings in the first ...
3 CitationsSource
#1Tao Zhou (Sichuan University)H-Index: 3
#2Jinhai Pan (Sichuan University)H-Index: 1
Last. Xin Zhou (Sichuan University)H-Index: 6
view all 12 authors...
Dental follicle cells (DFCs) are a group of mesenchymal progenitor cells surrounding the tooth germ, responsible for cementum, periodontal ligament, and alveolar bone formation in tooth development. Cascades of signaling pathways and transcriptional factors in DFCs are involved in directing tooth eruption and tooth root morphogenesis. Substantial researches have been made to decipher multiple aspects of DFCs, including multilineage differentiation, senescence, and immunomodulatory ability. DFCs ...
20 CitationsSource
Cited By0