Simultaneous qualitative and quantitative analyses of ursolic acid and oleanolic acid in Punica granatum L. (Pomegranate) flowers by high-performance thin-layer chromatography

The purpose of this study was to develop a method for the simultaneous qualitative and quantitative analysis of ursolic acid (UA) and oleanolic acid (OA) in Punica granatum L. flowers. UA and OA were analyzed qualitatively and quantitatively by high-performance thin-layer chromatography (HPTLC). The thin layers chosen were high-performance thin-layer G plates. Sample spots were pretreated in situ with iodine–dichloromethane solution with...