Biology

Pesticide

Chromatography

Gas chromatography–mass spectrometry

Orbitrap

Triple quadrupole mass spectrometer

Agronomy

Selected reaction monitoring

Gas chromatography

Pesticide residue

Analytical Chemistry (journal)

Mass spectrometry

Chemistry

Detection limit

Tandem mass spectrometry

Food Chemistry

Performances of multiresidue analysis of one hundred of pesticides and contaminants, using GC-Q-Orbitrap method in full scan mode were compared to those obtained with GC-triple-quadrupole method in multiple reaction monitoring mode. In terms of sensitivity, 86% of molecules exhibited lower limit of detection values using GC-Q-Orbitrap than using GC-triple-quadrupole. For the GC-Q-Orbitrap method, more than 85% of the pesticides and contaminants showed good recovery [70–120%] in wheat samples, with relative standard deviation values < 20%. GC-Q-Orbitrap method appeared the most sensitive for most pesticides studied in wheat with limit of quantification values ranged between 0.1 µg/kg and 4 µg/kg. Moreover, the matrix effect was acceptable in wheat extracts for 84 molecules but strong suppression of the chromatographic signal was observed for 16 molecules for the GC-Q-Orbitrap method. The injection of unpurified wheat extracts spiked at 10 µg/kg proved the potential of the GC-Q-Orbitrap method for use in performing high-throughput pesticide screening.

Comparison of new approach of GC-HRMS (Q-Orbitrap) to GC–MS/MS (triple-quadrupole) in analyzing the pesticide residues and contaminants in complex food matrices