ms ms

high performance liquid

traditional chinese

dietary supplements

phenolic acids

rat plasma

chromatography coupled

crude extracts

Journal of Pharmaceutical and Biomedical Analysis

• The chemical profile of YCWLP was revealed for the first time. • Totally 138 compounds were identified in YCWLP by the UPLC-FT-ICR-MS. • The fragmentation patterns of 6 types of ingredients were summarized. • This study provides a scientific basis for further quality control and pharmacology research. • This study provides a reference for finding active ingredients of other traditional Chinese medicines. Yinchen Wuling Powder (YCWLP) is a classic Chinese medicine prescription with a long history and has been commonly used for treating jaundice hepatitis, liver fibrosis, hyperlipidemia and early diabetes in clinical applications. However, the chemical composition of YCWLP is still unclear. In order to obtain the chemical profile of YCWLP, a systematic ultra-performance liquid chromatography coupled with fourier transform ion cyclotron resonance mass spectrometry (UPLC-FT-ICR-MS) method was developed in this study. As a result, a total of 138 compounds including terpenoid acids, organic acids, flavonoids, sesquiterpenes, coumarins and anthraquinones were identified by comparing the retention time, molecular ions and fragmentation behaviors with the reference compounds or the in-house database. This study comprehensively elucidated the chemical basis of YCWLP and provided a scientific basis for further quality control and pharmacology research.

Rapid characterization of the chemical constituents of Yinchen Wuling Powder by UPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry