Rapid characterization of the chemical constituents of Yinchen Wuling Powder by UPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Published on May 10, 2021in Journal of Pharmaceutical and Biomedical Analysis3.209
· DOI :10.1016/J.JPBA.2021.114022
Yumeng Zhang4
Estimated H-index: 4
(SPU: Shenyang Pharmaceutical University),
Min Zhao8
Estimated H-index: 8
(SPU: Shenyang Pharmaceutical University)
+ 3 AuthorsMiao Wang9
Estimated H-index: 9
(SPU: Shenyang Pharmaceutical University)
Abstract Yinchen Wuling Powder (YCWLP) is a classic Chinese medicine prescription with a long history and has been commonly used for treating jaundice hepatitis, liver fibrosis, hyperlipidemia and early diabetes in clinical applications. However, the chemical composition of YCWLP is still unclear. In order to obtain the chemical profile of YCWLP, a systematic ultra-performance liquid chromatography coupled with fourier transform ion cyclotron resonance mass spectrometry (UPLC-FT-ICR-MS) method was developed in this study. As a result, a total of 138 compounds including terpenoid acids, organic acids, flavonoids, sesquiterpenes, coumarins and anthraquinones were identified by comparing the retention time, molecular ions and fragmentation behaviors with the reference compounds or the in-house database. This study comprehensively elucidated the chemical basis of YCWLP and provided a scientific basis for further quality control and pharmacology research.
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