Self-assembling micellar system based on Pluronic and pyrazole-dithiocarbazate-conjugate stimulates production of nitric oxide from macrophages

Published on Mar 1, 2021in Colloid and Interface Science Communications4.914
· DOI :10.1016/J.COLCOM.2021.100378
Analu Rocha Costa2
Estimated H-index: 2
(State University of Santa Cruz),
Rodrigo Novais França1
Estimated H-index: 1
(State University of Santa Cruz)
+ 4 AuthorsRodrigo Luis Silva Ribeiro Santos2
Estimated H-index: 2
(State University of Santa Cruz)
Source
Abstract
Abstract Nitrogen and sulfur-containing heterocyclic compounds as pyrazole and dithiocarbazate have historically been reported as sources of therapeutic agents. This work reports by fluorescence measurements the colloid phenomena between the 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) and Pluronic F127, a copolymer that has a tendency to self-assemble into micelles in aqueous medium. The cytotoxicity of the DTC-based micellar system was evaluated in Swiss mice peritoneal macrophages, and the production of nitric oxide (NO) by these cells was also analyzed in vitro and molecular docking analysis. The DTC-based micellar system prevents the DTC decomposition and increases solubility. The Hofmeister ions modulate DTC-micelle interactions. The DTC-based micellar system is not cytotoxic to Swiss mice peritoneal macrophages and induces the production of NO by these cells. These findings make the DTC-based micellar system of great interest in health/disease processes, since its immunomodulatory action on the macrophages may be tools on further studies for the development of novel microbicide or anti-inflammatory drugs.
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Last. Rodrigo Luis Silva Ribeiro Santos (State University of Santa Cruz)H-Index: 2
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Dithiocarbazate Schiff bases have been reported as a class of compounds that exhibit a wide range of pharmacological activities against neglected diseases such as malaria, trypanosomiasis, and tuberculosis. This work reports the encapsulation of the 1-(S-benzyldithiocarbazate)-3-methyl-5-phenyl-pyrazole (DTC) into biodegradable polymeric nanoparticles (NPs) of poly(lactic acid) (PLA) aiming potential drug delivery application. The DTC-loaded PLA-NPs were prepared by the single-nanoemulsification...
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#1Analu Rocha Costa (UCSC: University of California, Santa Cruz)H-Index: 2
#2Thacilla Ingrid de Menezes (UCSC: University of California, Santa Cruz)H-Index: 2
Last. Rodrigo Luis Silva Ribeiro Santos (State University of Santa Cruz)H-Index: 2
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The aim of the study was to synthesize and characterize a new ruthenium(II)-pyrazole/dithiocarbazate complex derived from cis-[RuCl2(dmso)4] (Ru-dmso) and coordinated with 3-methyl-5-phenyl-pyrazoline-1-(S-benzyl)dithiocarbazate (dtc). The reaction of Ru-dmso with the dtc ligand was performed with equimolar amount, resulting in a neutral monosubstituted complex [RuCl2(dmso)3(dtc)] (Ru-dtc).To characterize this complex, conductivity measurements and spectroscopic techniques (1H-NMR, FTIR, UV–Vis,...
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#1Ekta Goshi (SZU: Shenzhen University)H-Index: 2
#2Gaoxin Zhou (SZU: Shenzhen University)H-Index: 6
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Initially being considered as an environmental pollutant, nitric oxide has gained the momentum of research since its discovery as endothelial derived growth factor in 1987. Extensive researches have revealed the various pathological and physiological roles of nitric oxide such as inflammation, vascular and neurological regulation functions. Hence, the development of methods for quantifying nitric oxide concentration and its metabolites will be beneficial to well know about its biological functio...
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#1Fernando Spiller (UFSC: Universidade Federal de Santa Catarina)H-Index: 17
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Abstract Nitric oxide (NO) is produced by enzymatic activity of neuronal (nNOS), endothelial (eNOS), and inducible nitric oxide synthase (iNOS) and modulates a broad spectrum of physiological and pathophysiological conditions. The iNOS isoform is positively regulated at transcriptional level and produces high levels of NO in response to inflammatory mediators and/or to pattern recognition receptor signaling, such as Toll-like receptors. In this review, we compiled the main contributions of our g...
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#2Ershuai Zhang (WSU: Wayne State University)H-Index: 6
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Micelles have been studied as drug delivery carriers for decades. Their use can potentially result in high drug accumulation at the target site through the enhanced permeability and retention effect. Nevertheless, the lack of stability of micelles in the physiological environment limits their efficacy as a drug carrier. In particular, micelles tend to disassociate and prematurely release the encapsulated drugs, lowering delivery efficacy and creating toxicity concerns. Many efforts to enhance th...
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#1Charlotte Farah (Cliniques Universitaires Saint-Luc)H-Index: 3
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Nitric oxide (NO) signalling has pleiotropic roles in biology and a crucial function in cardiovascular homeostasis. In this Review, Balligand and colleagues summarize the updated paradigms on NO synthase regulation, NO interaction with reactive oxidant species in specific subcellular compartments, and downstream effects of NO in target cardiovascular tissues under both physiological and pathological conditions.
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Last. Stefan Lorkowski (FSU: University of Jena)H-Index: 59
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Abstract The Griess assay is used to measure nitric oxide concentrations in liquid solutions after reaction into nitrite. The assay is challenging when applied to cell culture supernatants. During optimization, we focused on the anti-inflammatory potential of test compounds in murine RAW264.7 macrophages. This led to (i) the required inductivity of cells by lipopolysaccharide (LPS) and allowed (ii) the characterization of putative anti-inflammatory test compounds with high sensitivity. The modif...
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Cited By2
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#1Rebeca de Oliveira Costa (State University of Santa Cruz)H-Index: 1
#2Janclei Pereira Coutinho (State University of Santa Cruz)H-Index: 4
Last. Rodrigo Luis Silva Ribeiro Santos (State University of Santa Cruz)H-Index: 2
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Last. Teng Liu (Peking Union Medical College)H-Index: 3
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