Programmed cell death

Nanoparticle

Biology

MTT assay

Materials science

Apoptosis

Eriobotrya

JavaScript

HeLa

Biochemistry

Computer science

Botany

Silver nanoparticle

Immunology

Drug delivery

Nanotechnology

Japonica

Immune system

In vitro

Microbiology

Inflammation

Internal medicine

Zeta potential

Medicine

Chemistry

Programming language

Phagocytosis

DNA fragmentation

Cancer

Cancer cell

Artificial Cells Nanomedicine and Biotechnology

Nanoparticles (NPs) have biological activities like antibacterial, antifungal, drug delivery, immunomodulation and antitumor activities. The aim of the current study was to investigate some of biomedical applications of silver NP synthesis using extracts from leaves of Eriobotrya japonica. Colour changes, UV-visible spectroscopy, SEM, zeta potential, dynamic light scattering, FTIR and XRD were used to confirm AgNPs formation. The UV-vis spectrum absorption band was observed at almost 430 nm. The SEM image shows quasi-spherical shape of AgNPs. The zeta potential demonstrated the negative surface charge of NPs. FTIR results showed the functional groups of AgNPs. Crystalline nature of AgNPs was confirmed by XRD pattern. MTT assay was used to study the anti-proliferative activity against MCF-7 and HeLa cells. Apoptosis was tested using a DNA-fragmentation test, and expression of P53. AgNPs inhibited the proliferation of MCF-7 and HeLa cells, and reduced inflammation. Treatment with AgNPs significantly decreased allergic disorder. AgNPs stimulated the phagocytosis process in BMDMs. The results suggested that AgNPs could be a promising therapy for future and preventing inflammation, reduce allergic disorders and prevent bacterial infection through the up-regulation of phagocytosis. Hence, future work such as developed and improved NPs as adjuvants, immune-modulating substances and nano-drug delivery system is needed.

Green synthesis of silver nanoparticles from <i>Eriobotrya japonica</i> extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction