Metal

Gene

Coronavirus

Adsorption

Electrochemiluminescence

Photochemistry

Materials science

Chromatography

Biochemistry

Coronavirus disease 2019 (COVID-19)

Physical chemistry

Peroxydisulfate

Combinatorial chemistry

Physics

Nanotechnology

Organic chemistry

Porphyrin

Luminescent Measurements

Luminescence

Porcine epidemic diarrhea virus

Infectious disease (medical specialty)

Metal-organic framework

Potassium

Nanomaterials

Medicine

Chemistry

Electrode

Detection limit

Disease

Optoelectronics

Pathology

Analytical Chemistry

The sensitive detection of coronavirus is of vital importance for the prevention of its rapid spread. Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea and death in neonatal piglets. In this work, a novel PCN-224-based electrochemiluminescence (ECL) system was constructed for PEDV detection with high sensitivity. We found that PCN-224 can be employed as an ECL reporter with a strong signal because of its zirconium-based organic porous frame nanomaterial with a large specific surface area and stable structure. TiO2 nanoparticles were used as an accelerator for the first time to promote the reduction of coreactant potassium peroxydisulfate on the cathode; thus, the initial ECL signal of PCN-224 was significantly amplified. In the presence of PEDV, the ECL signal decreased due to the block effect to electron transfer. As a result, the novel "signal off" biosensor achieved a sensitive detection of PEDV ranging from 1 pg/mL to 10 ng/mL, with a detection limit of 0.4 pg/mL (S/N = 3). Importantly, the PCN-224 nanomaterial enriched the ECL system in biological analysis, and the proposed strategy provided a new route for coronavirus detection.

Novel Porphyrin Zr Metal–Organic Framework (PCN-224)-Based Ultrastable Electrochemiluminescence System for PEDV Sensing