Development and evaluation of poly adenosine 5′‐diphosphate‐ribose polymerase 1 immobilization‐based receptor chromatography

Yanghe decoction is a traditional Chinese medicine prescription and has been used for breast cancer treatment for many years. However, the effective ingredients in the decoction have not been identified. The expression of poly(ADP‐ribose) polymerase‐1 is highly related to breast cancer. Using poly(ADP‐ribose) polymerase‐1 as a probe, we expressed the haloalkane dehalogenase‐tagged protein in BL21(DE3) E. coli , immobilized it on...