Biomedical engineering

Chemistry

Materials science

Cell biology

Anatomy

Membrane

Biology

Biomineralization

Mesenchymal stem cell

Periosteum

Paleontology

Medicine

Regeneration (biology)

Biochemistry

Bone healing

Biomaterials

Periosteum plays a pivotal role in vascularization, ossification and remodeling during the healing process of bone injury. However, there are few studies focused on the construction of artificial implants with periosteum-mimetic effect. To emulate the primary role of natural periosteum or endosteal tissues in bone regeneration, here we provide a functional biomimetic membrane with micropatterns of site-speciﬁc biomineralization. The micropattern is generated by using printed hydroxyapatite nanoparticles (HANPs), combined with selective growth of biomineralized apatite and in situ coprecipitation with growth factors. The biomimetic membrane can sustainably provide a periosteum-mimetic microenvironment, such as long-term topographical guidance for cell recruitment and induced cell differentiation, by releasing calcium phosphate and growth factors. We demonstrated that rat mesenchymal stem cells (rMSCs) on such biomimetic membrane exhibited highly aligned organization, leading to enhanced angiogenesis and osteog...

Bioinspired membrane provides periosteum-mimetic microenvironment for accelerating vascularized bone regeneration