Abstract This work aims to study the bio-interfacial properties of poly(ester-urethane) composite scaffolds based on poly (3-hydroxybutyrate) [PHB] and modified by surface plasma-radiation-induced graft polymerization of N-isopropylacrylamide (NIPAAm). The delivering power of the Argon radio-frequency plasma was increased from 5−20 W. The yields over the polyurethane surface, grafting density, topographical dimensions, architecture, surface energy, and wettability were surveyed in the established plasma regimes. Successful grafting of NIPAAm was verified by Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis. Characterization using atomic force microscopy revealed that the surface of the scaffolds is more uniform in lower plasma delivering powers. The results show a high grafting density surface with a low degree of grafting (3–9 %). The contact angles decreased, and the surface energy rose with increasing delivering power. The surface area, total pore volumes are similar between the samples. The porosity percentage and the average pore diameter hinted at a decrease in the treated composite as compared to the pristine scaffold. Although all the composites showed to be non-toxic and highly viable (>98 %) for human dermal fibroblasts, the results showed better attachment, spreading, and growth of fibroblasts on scaffolds with moderate hydrophilicity (5 W). The in vivo experiments revealed that the plasma-treated scaffolds diminished the inflammatory infiltrate, which means an improved acceptance as compared with untreated PHB polyurethane.
Abstract The synthesis of the poly(ester-urethanes) using poly(3-hydroxybutyrate) has gained increasing interest as scaffolds for use in tissue engineering despite their moderate hydrophobicity and biocompatibility. However, it is not yet known if the grafting of poly(N‐hydroxyethyl acrylamide) is suitable to improve the degree of hydrophilicity of the surface. This study aims to tailor the surface of these biopolymers by plasma-induced graft polymerization of N‐hydroxyethyl acrylamide. The synt...
Abstract Both chitosan (CS) and poly(3-hydroxybutyrate) (P(3HB)) are two good options for use in tissue engineering. Some of the advantages of these polymers are biodegradability and non-toxicity. However, the inadequate mechanical properties and hydrophobicity (P3HB) and the low porosity (CS) are examples of shortcomings that restrict its applications. The polysaccharide was grafted onto P(3HB) in a previous work for removing these disadvantages. As of yet, it is still unknown whether our P(3HB...
Last. Yuanyuan Gao(SDNU: Shandong Normal University)H-Index: 2
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Abstract The aim of this work is to provide a new kind of polyurethane with improved surface blood compatibility for long-term blood-contacting biomaterials. In the study, an aliphatic poly(ester-urethane) (H-PEU) with uniform-size hard segments was synthesized by one-step chain extension of poly(e-caprolactone) (PCL) with diurethane diisocyanate (HBH), and biomimetic phosphorylcholine (PC) groups were immobilized onto the film surface with high grafting efficiency by three-step chemical treatme...
: Twenty-six different bacterial strains were isolated from samples taken from different locations Dammam, Saudi Arabia, for screening of their polyhydroxyalkanoate (PHA) production capability. The initial screening was conducted by staining with Sudan Black B and Nile Red, followed by examination under fluorescence and electron microscopes to characterize PHA granule formation. The PHA-producing bacterial isolates were identified using 16S rRNA gene analyses; the most potent bacterial strain wa...
Abstract In this work, a green composite from poly(3-hydroxybutyrate), or P(3HB), grafted with 2-amino-ethyl methacrylate, hydroxylated resin and silica nanoparticles was developed. The goal was to determine if the new poly(urethane-urea) composite can work as an appropriate substrate for biological applications. The mechanism for the polyurea step-growth polymerization was proposed. The scaffolds showed an average porosity and degree of swelling of 55% and 7%, respectively, as well as a reinfor...
Abstract The chemical modification of biodegradable poly(3-hydroxybutyrate) (PHB) is useful for biomedical applications. In this study, the transesterification reaction of PHB was carried out under reflux conditions in the presence of 1,4-butanediol to form telechelic PHB-diol. Further modification of PHB-diol into PHB-diacrylate was carried out by the reaction of PHB-diol with acryloyl chloride. PHB-diacrylate was grafted with amino compounds such as 1,4-butanediamine, 1,3-propanediamine, 1,2-e...
#1Liu Lulu(SDNU: Shandong Normal University)H-Index: 1
#2Yuanyuan Gao(SDNU: Shandong Normal University)H-Index: 1
Last. Zhaosheng Hou(SDNU: Shandong Normal University)H-Index: 6
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: In the paper, poly(ethylene glycol) (PEG) was grafted on the surface of poly(ester-urethane) (SPEU) film with high grafting density for biomedical purposes. The PEG-surface-grafted SPEU (SPEU-PEG) was prepared by a three-step chemical treatment under mild-reaction conditions. Firstly, the SPEU film surface was treated with 1,6-hexanediisocyanate to introduce -NCO groups on the surface with high density (5.28 × 10-7 mol/cm²) by allophanate reaction; subsequently, the -NCO groups attached to SPE...
Last. Kyung-Jin Kim(KNU: Kyungpook National University)H-Index: 26
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Polyhydroxyalkanoates (PHAs) are diverse biopolyesters produced by numerous microorganisms and have attracted much attention as a substitute for petroleum-based polymers. Despite several decades of study, the detailed molecular mechanisms of PHA biosynthesis have remained unknown due to the lack of structural information on the key PHA biosynthetic enzyme PHA synthase. The recently determined crystal structure of PHA synthase, together with the structures of acetyl-coenzyme A (CoA) acetyltransfe...