Hierarchical Self-Assembly Mechanism of Ladder-Like Orientated Aβ40 Single-Stranded Protofibrils into Multistranded Mature Fibrils

The complex self-assembly processes in three dimensions of Alzheimer’s β-peptide (Aβ) amyloid protofibrils into polymorphic mature fibrils, particularly the relative protofibril orientation and packing mechanism, are poorly understood. We report here the identification and quantification of the hierarchical self-assembly details among distinct Aβ40 fibrils, particularly the winding pictures of two, three, and four individual single-stranded...